摘要
利用玉米原料酒精浓醪发酵产生的酒糟,对其培养热带假丝酵母生产SCP进行了初步的研究。经过优化后的发酵条件为:酒糟中添加0.2‰CaCl2和1.0‰KH2PO4,起始pH为4.5,装液量为30mL/500mL三角瓶;发酵26h,获得的最高菌浓为11.3亿/mL;发酵过程中酵母基本消耗完酒糟中的糖,同时还消耗了酒糟中的有机酸。发酵得到的SCP样品中含水分9.47%,粗蛋白47.73%,真蛋白45.09%,取得了较好的效果。
Single Cell Protein （SCP） was produced from distiller＇s grains, which came from high gravity ethanol fermentation of corn, by using Candida tropicalis in shake-flasks. The optimum conditions of fermentation were as follows： CaCl2 0.2 %c, KH2PO4 1.0 ‰, initial pH value as 4.5,and the cultivation performed in 500 mL shake flask containing 30 mL medium. Under the above conditions, 1,130 million/mL C. tropicalis was obtained. HPLC analysis indicated that C. tropicalls consumed almost all the sugar, and organic acids were also consumed. In result, satisfactory results were achieved and the produced SCP samples contained 9.47 % water, 47.73 % crude protein and 45.09 % true protein.
出处
《酿酒科技》
北大核心
2007年第12期95-98,共4页
Liquor-Making Science & Technology
基金
国家科技部"十五"攻关项目资助(2001BA501A01)
关键词
热带假丝酵母
单细胞蛋白
酒糟
酒精浓醪发酵
Canclida tropicalis
single cell protein
distiller＇s grains
high gravity ethanol fermentation
