摘要
目的探讨ｐ２１Ｈ－ｒａｓ表达水平及第１２位密码子突变与宫颈鳞状上皮内病变（Ｓｑｕａｍｏｕｓｉｎ－ｔｒａｅｐｉｔｈｅｌｉａｌｌｅｓｓｉｏｎ，ＳＩＬ）、宫颈癌的关系及预后价值。方法同时用ＡＢＣ免疫组织化学、两步ＰＣＲ－ＲＦＬＰ方法对２９例慢性宫颈炎、６８例ＳＩＬ及１７１例宫颈癌石蜡包埋组织进行ｐ２１Ｈ－ｒａｓ表达及Ｈ－ｒａｓ第１２位密码子突变检测。结果（１）ｐ２１Ｈ－ｒａｓ过度表达率和３＋强过度表达率依ＬＳＩＬ分别为２５．０％、０．０％，ＨＳＩＬ分别为６４．６％、３５．４％和宫颈癌分别为６６．１％、４９．７％顺序递增。（２）宫颈癌Ｈ－ｒａｓ第１２位密码子突变仅出现在Ⅱ、Ⅲ期中，突变率分别为２７．０％、５２．５％。有突变宫颈癌患者的５年存活率（２０．３％）显著低于无突变患者（７９．７％），且与淋巴结转移关系密切。结论ｐ２１Ｈ－ｒａｓ表达水平有助于宫颈癌的早期诊断，一旦Ｈ－ｒａｓ第１２位密码子突变发生，宫颈癌的恶性侵袭性行为显著增高，可作为判断预后的一项指标。
Objective To study the clinical significance of p21 H ras expression and H ras codon 12 mutation in SIL and cervical carcinoma. Methods p21 H ras expression and H ras codon 12 mutation was detected in the same paraffin embedded tissues of 171 cases of cervical carcinoma, 68 cases of SIL, and 29 cases of chronic cervicitis, by using immunohistochemical and PCR RFLP techniques.Results (1)p21 H ras was over expressed in 25.0% of the cases with low grade SIL but in most of them the immunohistochemical staining was not strong (score<3). p21 H ras over expression was present in 64.6% of the cases with high grade SIL and in 35.4% of them, the staining was strong (score=3). Even higher frequency of p21 H ras over expression was seen in cases with cervical carcinoma (66.1%) and in about one half (49.7%) the staining was strong. (2) H ras codon 12 mutation was only detected in stages Ⅱ and Ⅲ cervical carcinoma, with frequency rate of 27.0% and 52.5% respectively. The five year survival rate of patients with H ras codon 12 mutation (20.3%) was significantly lower than that without mutation (79.7%). Besides, there was a correlation between lymph node metastasis and H ras codon 12 mutation. Conclusion p21 H ras expression is helpful for early detection of cervical carcinoma. Aggressive biological behavior of cervical carcinoma is significantly increased once the H ras codon 12 mutation occurs. H ras codon 12 mutation is helpful to judge prognosis of cervical carcinoma.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1997年第4期306-307,共2页
Chinese Journal of Oncology
关键词
宫颈肿瘤
鳞状上皮病变
P21^(ras)
密码子
Uterine neoplasms Cervical intraepithelial neoplasia Proto oncogen protein p21 (ras) Codon Mutation
