摘要
[目的]通过观察加参方对充血性心力衰竭(CHF)大鼠肾脏水通道蛋白(AQP)mRNA表达的影响,探讨加参方改善水钠潴留的机制。[方法]采用结扎大鼠冠状动脉前降支法造成心肌梗死后形成慢性CHF模型,随机将CHF大鼠分为加参方组、模型组和假手术组共3组,每组8只。连续给药4周。[结果]加参方能够减少CHF大鼠异常增加的抗利尿激素(AVP)水平,抑制AVP-2R受体的激活,降低CHF大鼠肾脏内髓AQP-2mRNA表达。[结论]加参方通过调控CHF大鼠肾脏水通道蛋白AQP mRNA表达,使管腔液中的水通透性减少,减轻CHF时水钠潴留,促进尿液排泄,降低心脏容量负荷,可能是加参方增强心功能的机制之一。
[Objective] To observe the effect of Jiashen prescription on the expression of AQP mRNA of CHF rats and explore the effect mechanism to improve water-sodium retention. [Methods] CHF（congestive heart failure） after myocardial infarction induced by ligation of anterior descending coronary method in rats was established. They were randomly divided into three groups： Jiashen prescription group, model group and sham operated group. [Results] Jiashen prescription may decrease unusual increasing level of AVP and AQP-2m_RNA expression of CHF rats and inhibit AVP-2R activation. [Conclusion] Jiashen prescription may regulate AQP- 2mRNA expression of CHF rats, decrease water permeability of luminal liquid and water-sodium retention, promote evacuation of urine and cut down the volume load of heart. The effect mechanism of Jiashen prescription is probably related to strengthening heart function.
出处
《天津中医药大学学报》
CAS
2007年第4期188-191,共4页
Journal of Tianjin University of Traditional Chinese Medicine
基金
天津市应用基础研究计划面上项目(06YFJMJC09700)
天津市卫生局中医
中西医结合科研专项资金项目(2005064)。
