摘要
用免疫组织化学ＡＢＣ法，显示绍鸭胚后发育期间胰内高血糖素细胞（Ａ细胞），观察Ａ细胞的形态及分布；用显微图像分析仪测定Ａ细胞的积分光密度值；并求各时期Ａ细胞的平均积分光密度值，再作统计学分析。结果表明：Ａ细胞形态多样，但以多面形为主，并具多种形式的胞质突起。常成群散在分布于胰岛内，少数零散位于胰腺中。Ａ细胞总积分光密度值随年龄而变化：７～４２日龄为生长发育峰期，以后呈波浪式缓慢下降趋势，其中７～１４日龄为增长高峰期。本文显示了胰腺Ａ细胞在胚后发育期间的变化规律。为胃肠胰内分泌系统的功能研究提供动态变化的基础资料。
Glucagon cells (A cell) in the splenic lobi of pancreas of Shao ducks were studied with ABC immunohistochemical method during their postnatal development.First the IOD(integrate optical density) of A cells were measured with image analysis and then statistically analyzed.The results showed that A cells were mainly polygonal with various cytoplasmic processes.Most of A cells were distributed in a loose group in the pancreatic islets;a few of them were in the exocrine part of the pancreas.There was a marked variation of IOD of A cells with days of age.This paper provided basic datum for studying the dynamic change of the gastroenteropancreatic endocrine system.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1997年第5期428-433,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金
关键词
绍鸭
胰腺A细胞
生长发育
免疫组织化学
鸭
Shao duck,Pancreas A cell,Development,Immunohistochemistry,Image analysis
