摘要
目的对参附注射液抗心肌缺血/再灌注损伤可能的分子机制进行初步探讨。方法SD大鼠19只,分为2组,对照组和参附注射液组,分别为生理盐水组,心肌缺血30 min/再灌注10 min+参附注射液组。建立心肌缺血/再灌注损伤模型,分别收集各组大鼠心肌组织,提取心肌细胞总RNA,利用Affymetrix Rat 230A芯片进行基因差异表达分析,用Microarray Suite 5.0软件读取并进行数据处理。结果心肌缺血/再灌注10 min后,给药组与对照组比较明显上调基因222条,明显下调基因246条。与心肌缺血/再灌注损伤密切相关的主要基因有,超氧化物岐化酶、谷胱苷肽S转移酶、巨噬细胞移动抑制因子、热休克蛋白、钙通道电压依赖相关基因及金属硫因等基因。这些基因主要与抗氧自由基损伤、抑制细胞凋亡、心肌保护、抑制炎症及抑制心肌缺血/再灌注损伤时的钙超载等机制相关。结论参附注射液对心肌起到保护作用的分子机制主要是通过调节抗氧自由基损伤相关基因、炎症相关基因及钙转运酶等相关基因,进而参予调控心肌缺血/再灌注损伤疾病过程中细胞信号转导。通过抗过氧化脂质损伤作用,减轻缺血时被激活的白细胞聚集等炎症反应,抑制缺血/再灌注期间钙超载所致的细胞凋亡发生,从而提高心肌细胞的防御能力,起到保护心肌的作用。
OBJECTIVE The goal of this study was to explore the possible mechanism of the protection provided by Shenfu injection against ischemia/reperfusion -induced myocardial injury. METHODS This study established the model of myocardial ischemia/ reperfusion injury. In this model, rats were divided into two groups, control group and Shenfu injection group. There are myocardial ischemia for 30 min reperfusion for 10 min ＋ NS groups and myocardial ischemia for 30 min reperfusion for 10 min ＋ Shenfu Injection groups. In the study, we collected the rat＇s heart tissue of each group, extracted mRNA from cardiomyocyte and used the gene chip of Affymetrix Rat 230A to observe and analysis expression of gene, and then loaded and processed the lab data using Microarray Suite 5.0. RESULTS Compare the group Myocardial ischemie/reperfusion for 10 min in the control and the Shenfu injection groups, there ware 222 genes in Shenfu injection group were obviously increased, and 246 genes were obviously decreased. The genes that have a close correlation with SI/RO-induced myocardial injury were： Superoxide dismutase 1, soluble, glutathione S-transferase omega 1, Macrophage migration inhibitory factor, Heat shock 27 Kda protein 1, Calcium channel, voltage-dependent, L type and Metallothionein （MT） et al. These genes were mainly are related to antioxygen free radical injury ,preventing apoptosis ,cardioprotective effects ,anti-inflammation and preventing Ca^2＋ over loading during the period of ischemia injury. CONCLUSION The molecule mechanism of Shenfu injection that protects cardiomyocytes against ischemia/reperfuslon injury by （ 1 ） adjusting the signal transfer of cell during the ischemia/reperfusion -induced myocardial injury, （2） Simuhaneity Shenfu injection are regulating the gene expression of correlation genes, （3） the effect of anti-lipid peroxides injury. As a result of inhibite the gathering leucocyte, Shenfu injection reduced ischemia/ reperfusion injury. Shenfu injection can prevent the apoptosis of ca
出处
《中国药学杂志》
CAS
CSCD
北大核心
2007年第23期1779-1783,共5页
Chinese Pharmaceutical Journal
基金
卫生部999中药注射液科研基金(200307)
