摘要
以亲缘关系极近的新高系梨10个品种及被视为‘新高’梨亲本的‘天之川’和‘今村秋’梨等为试验材料,对其基因组DNA进行了PCR-RFLP检测、DNA序列测定及生物信息学分析。结果表明:新高系梨10个品种即‘新高’、‘黄金’、‘水晶’、‘早生黄金’、‘早蜜新高’、‘天皇’、‘金秋’、‘晚大新高’、‘农家新高’和‘鲜黄’的S基因型分别为S3S9、S3S4、S3S9、S3S4、S3S9、S3S9、S3S9、S3S9、S3S9和S3S5;‘天之川’、‘今村秋’的S基因型分别为S1S9和S1S6,通过基因型判定‘今村秋’非‘新高’梨的父本。
Ten cultivars with extremely close relationship from Niitaka line pear were used as materials for S-genotyping. In addition, two cultivars ‘Imamuraaki＇ and ‘Amanogawa＇ that were considered to be the parents of ‘Niitaka＇ were also included. PCR - RFLP analysis of genomic DNAs of the 12 cuhivars, nucleotide sequencing of amplified fragments and sequence analysis were performed. As the results, the ten cuhivars of Niitaka line were genotyped as follows ： ‘Niitaka＇ （ S3 S9 ）, ‘Whang Keum Bae＇ （ S3S4 ）,‘Sujeong＇ （S3S9 ）, ‘Joseng Whang Keum＇ （S3S4 ）, ‘Soumi Tuniitaka＇ （ S3S9 ）, ‘Yewang＇ （ S3 S9 ）, ‘Jinqiu ＇ （S3S9） , ‘Banndai Niitaka＇ （S3S9） , ‘ Noka Niitaka＇ （S3S9 ）, and ‘Sunhwang＇ （S3S5 ）. The S-genotype of ‘Amanogawa＇ and ‘Imamuraaki＇ was identified as SIS9 and SIS6, respectively, suggesting that ‘Imamuraaki＇ is unlikely to be the pollen parent of ‘Niitaka＇.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第6期1353-1360,共8页
Acta Horticulturae Sinica
基金
国家林业局重点项目(2006-12)
国家‘948’项目(2001-36)
关键词
梨
砂梨
新高系梨
自交不亲和性
S基因型
鉴定
Pear
Pyrus pyrifolia
Niitaka line pear
Self-incompatibility
S-genotype
Identification
