摘要
目的研究缺氧诱导丝裂原因子(hypoxia-induced mitogenic factor,HIMF)在先天性膈疝(congenital diaphragmatic hernia,CDH)胎鼠肺组织中的表达,探讨其在CDH肺发育不良中的作用。方法实验组10只BABL/C小鼠妊娠8 d时经胃管注入25 mg除草醚,正常对照组给予食用油,妊娠21 d行剖腹产,解剖胎鼠两侧肺组织,采用免疫组化、Western blot方法检测HIMF表达。结果实验组CDH致畸率56.5%,肺发育不良,处于假腺体期和原始肺小管期,HIMF蛋白显著表达下调(P<0.05);实验组内产生CDH者胎肺内HIMF表达水平与无CDH者比较差异无显著性意义(P>0.05)CDH膈疝侧与非膈疝侧肺组织HIMF表达差异无显著性意义(P>0.05)。结论在CDH肺发育不良组织中HIMF蛋白表达显著下调,且早于膈疝形成,可能参与CDH肺发育不良的发病机制。
Objective To study the expression of hypoxia-induced mitogenic factor （HIMF） in fetal lungs of mice and its influence on pulmonary hypoplasia in congenital diaphragmatic hernia （CDH）. Methods CDH was induced in ten BABL/ C pregnant mice on 8th day of gestation with a single dose of nitrofen （25 mg）. via gastric tube. In a control group oil was inserted into the stomach. All fetuses were delivered by cesarean delivery on day 21. Lung tissues were harvested for HIMF expression by immunohistochemistry staining and Western blot method. Results CDH was found with an incidence of 56. 5% of mice fetuses. The lungs showed marked hypoplasia mainly arrested at pseudoglandular and canalicular stages with significantly down- regulated HIMF expression （P〈0. 05）. There was no significant difference in HIMF expression between the lungs of CDH and those without （P〉0. 05）, as well as no difference between the lungs from hernia side and contralateral side （P〉0. 05）. Conclusions Down regulation of HIMF protein is present in pulmonary hypoplasia of CDH and earlier than the formation of diaphragmatic hernia, which may participate in the pathogenesis of pulmonary hypoplasia of CDH.
出处
《中华小儿外科杂志》
CSCD
北大核心
2007年第11期594-597,共4页
Chinese Journal of Pediatric Surgery
基金
国家自然科学基金(No.30772359)
教育部新世纪优秀人才支持计划(NCET-06-0641)
关键词
疝
横膈
先天性
肺发育不良
缺氧诱导丝裂原因子
Hernia, diaphragmatic, congenital
Pulmonary hypoplasia
Hypoxia-induced mitogenic factor
