摘要
目的建立高效液相色谱-荧光分析(HPLC-FLD)测定人血浆中替米沙坦质量浓度的方法。方法色谱柱为AgilentHCC18柱(250mm×4.6mm,5μm),柱温30℃,流动相为磷酸二氢钾缓冲液(磷酸调节pH值为3.7)-甲醇(29∶71),流速1.0mL/min,荧光激发波长为300nm,发射波长为366nm。结果替米沙坦和萘普生(内标)的保留时间分别为5.7min和4.4min;替米沙坦质量浓度范围是2.42～484ng/mL(r=0.9997),最低定量质量浓度为2.42ng/mL。结论该法操作简便、选择性好、灵敏度高,可用于替米沙坦的血药浓度测定和药代动力学研究。
Objective To establish a HPLC method for the determination of the telmisartan concentration in human plasma. Methods Using naproxen as internal standard,purified plasma samples were analyzed by a HPLC consisted of a Agilent HC- C18 （250 mm×4. 6 mm, 5 μm） column with the maintenance temperature at 30℃. The mobile phase was 0.01 mol/L potassium dihydrogen phosphate solution-methanol （29：71） at the flow rate of 1.0 mL/min. The fluorescence detector was set at λE 300 rim, λEm 366 nm. Results The drug blank plasma did not interfere with the determination of drugs and internal standard. A good linear relationships was observed from 2.42-484 ng/mL （r=0. 999 8）, the limit of quantitative was 2. 42 ng/mL. Conclusion This method is simple, selective and sensitive. It is shown to be suitable for clinical monitoring concentration of telmisartan in human plasma and pharmacokinetic studies of telmisartan in humans.
出处
《中国药业》
CAS
2007年第23期48-49,共2页
China Pharmaceuticals
