摘要
目的:运用单纯疱疹病毒腺苷激酶/更昔洛韦(HSV-TK/GCV)自杀基因系统治疗模型动物并观察其疗效。方法:常规培养携带HSV-TK基因的包装细胞PA317,测定病毒滴度。在移植瘤内多点注射PA317细胞,观察更昔洛韦(GCV)治疗后的肿瘤体积变化,对移植肿瘤进行组织病理分析,PCR检测TK基因在移植肿瘤组织的转导情况。结果:重组逆转录病毒滴度为6×104cfu/ml,当肿瘤体积较小,应用HSV-TK/GCV系统治疗后肿瘤生长受到抑制(P<0.05);当肿瘤体积较大时,则抑制作用不明显;经PCR检测证实,TK基因能在肿瘤组织中转导。结论:HSV-TK/GCV系统对人难治性卵巢癌荷瘤裸鼠具有一定的治疗作用,但当肿瘤体积较大时,疗效不佳。
Objective：To observe the curative effects of HSV-TK/GCV system in the model of xenograft tumor of human refractory ovarian carcinoma. Methods：Retroviral vector containing HSV-TK gene was transfected into PA317 packaging cell, then these PA317 cells were cultivated conventionally. Retroviral vectors were then titrated. VPCs were injected at multiple sites of xenograft tumors. After independent therapy of HSV-TK/GCV, the volume of tumors were observed, then we used PCR to detect TK gene in the xenograft tumors. Results：The titer of retroviral is 6×10^4. HSV-TK/GCV system restrained the growth of xenograft tumors（ P 〈 0. 05 ）. It＇s difficult for the HSV-TK/GCV system to restrain the growth of big tumors independently. PCR confirmed that TK gene had been transferred into the xenograft tumor. Conclusion：There was independent effect of HSV-TK/GCV system. The effect of TK gene therapy was poor when the tumors＇ volume was obvious bigger.
出处
《临床肿瘤学杂志》
CAS
2007年第11期810-813,共4页
Chinese Clinical Oncology
基金
江苏省科技厅社会发展项目资助课题(bs2002058)
