摘要
目的:建立人卵巢癌SKOV3荷瘤鼠模型,通过检测扶正消瘤颗粒对SKOV3荷瘤鼠的抑瘤作用以及对免疫系统功能的影响,来研究该药对卵巢癌的抗肿瘤作用。方法:人卵巢癌SKOV3细胞皮下接种于40只小鼠背部,3周后成荷瘤鼠模型。分组给药,各组分别灌胃给药(ig)0.25mL连续10天。用碳粒廓清法检测扶正消瘤颗粒对动物模型网织内皮细胞系统的影响,并用流式细胞仪检测其对模型鼠NK细胞活性的影响。结果:(1)肿瘤细胞接种后1周即可触及肿瘤结节,3周后瘤体积达(107.7±20.1)mm3,给药后扶正消瘤颗粒3个剂量组的抑瘤率均明显高于模型组(P<0.01);(2)给药10天后3个剂量组K值均有非常明显性提高,与模型组相比具有统计学意义(P<0.01);(3)流式细胞仪检测可见,NK细胞的标志分子在模型组明显低于空白对照组(P<0.05),中剂量和大剂量组各标志均显著高于模型组(分别为P<0.05和P<0.01)。结论:扶正消瘤颗粒对人卵巢癌SKOV3荷瘤鼠模型具有抑瘤作用,并可提高网织内皮细胞系统和NK细胞系统的活性。
This paper studies the inhibitory effects of Fuzhengxiaoliu granula on ovarian cancer by developing a human ovarian cancer SKOV3 animal model. It also analyzes the associated changes of tumors and immune system. In the study, SK- OV3 cells were injected into the backs of BalB/C mice to develop animal models. Drugs were administrated at a dosage of 0. 25ml per animal for 10 days. Carbon particle clearance assay was applied to study the change of endothelial cell system, while flow cytometer was used to detect natural killer cells＇ activities. The study has obtained the following results： 1 ） Tumor nodes could be touched after lw and grown to 107.7 ± 20.1 （mm^3） ,without significant differences between all groups. Tumor inhibitory rates were higher in three groups of Fuzhengxiaoliu granula, compared with the model group; 2） 10d after administration, K value in 3 groups increased significantly, compared with the model controls（P 〈 0.01 ） ; 3） FCM findings showed that NK cells markers in the model group was much lower than the blank controls（P 〈 0.05） , while the markers in the middle and high dose groups were higher than that in the model group （P 〈 0.05 and P 〈 0.01 ）. It is concluded that Fuzhengxiaoliu granula has an inhibitory effect on human ovarian cancer models, and is able to enhance the endothelial cell system and natural killer cells＇ activities.
出处
《世界科学技术-中医药现代化》
2007年第5期49-53,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家中医药管理局专项基金(04-05JP22):中药扶正消瘤颗粒对卵巢癌化疗减毒增效及抗肿瘤机理的研究
关键词
扶正消瘤颗粒
卵巢癌
抑瘤作用
内皮细胞
NK细胞
Fuzhengxiaoliu granula
ovarian cancer
tumor inhibition
endothelial cell
NK cells
