期刊文献+

干旱胁迫下水稻叶片光合速率与叶绿素含量的相关性及其基因定位 被引量:14

Correlation Analysis and Gene Identification for Chlorophyll Content and Photosynthetic Rate in Rice Leaves under Drought Stress
下载PDF
导出
摘要 以水稻重组自交系珍汕97B×IRAT109 F9代群体195个株系为材料,用213个简单重复系列(SSR)标记构建了基于该群体的连锁图谱,对水稻叶片叶绿素含量和光合速率在干旱和正常条件下的数量性状位点(QTL)和双基因互作进行了分析,同时分析了叶绿素含量与光合速率的相关关系.结果表明:叶绿素含量与光合速率在正常供水下呈极显著正相关(r=0.1857,表示在1%水平上显著),但在干旱下则表现无关(r=0.0766).控制叶绿素含量的基因很复杂,主效QTL有13个,位于1、2、3、4、5、6、10号染色体上;其中,在干旱处理下检测到的主效QTL有6个,位于1、2、3、4、5号染色体上;在正常供水下检测到的主效QTL有7个,位于2、3、4、6、10号染色体上.在干旱和正常条件下它们分别解释了47.39%和56.19%的表型变异;在2种处理下均检出的主效QTL是2、3、4号染色体上的qCC2a、qCC2b、qCC3a、qCC3c、qCC4a、qCC4b;它们位于同一染色体的相同区段.在干旱和正常条件下检测到4个QTL与光合速率有关;其中干旱下有3个(qPR2、qPR10、qPR11),正常条件下1个(qPR10).它们分别被定位于2、10、11号染色体,共解释13.94%的表型变异.叶绿素含量互作效应位点有16对,涉及除10号染色体外的所有染色体;干旱下,有4对互作基因,共解释18.57%的表型变异,分别位于1-7、2-4、5-8、6-12号染色体上;正常供水下,有12对互作基因,共解释38.49%的表型变异,分别位于1-3、1-4、1-8、2-4、2-5、3-5、4-11、4-12、5-9、7-12、8-11号染色体上,其中3-5号染色体不同区段上有两对互作效应位点. The relationships of chlorophyll content ( CC ) and photosynthetic rate ( PR ) of rice leaves and their main-QTLs were studied by using a population of recombinant inbred lines (RILs) (Zhenshan97B ×IRAT109 ) in water gradient condition. There was significant correlation between CC and PR (R = 0. 185 7 ) in well watered condition, but no correlation in drought stress ( r = 0.076 6). A total of 13 main effects of QTLs locating on chromosomes 1,2, 3,4, 5,6 and 10 were found to associate with CC, which included six QTLs locating on chromosomes 1,2, 3,4, 5 in drought stress, and seven QTLs locating on chromosomes 2,3,4,6, 10 in normal condition. They explained 47.39% and 56.19% phenotypic variation, respectively, in drought and normal conditions. Six QTLs of them (qCC2α, qCC2b, qCC3α, qCC3c, qCC 4α, qCC4b) detected in both conditions were mapped on chromosomes 2,3 and 4. In the same time, 4 main effects of QTLs related to PR were located on chromosomes 2, 10 and 11 respectively with accounting total 13.94% phenotypic variation. They included three QTLs (qPR2, qPR10, qPR11) in drought stress and one QTL in normal (qPR10). A total of 16 pairs of epistatic QTLs of CC were detected on all chromosomes except chromosome 10. Four pairs of them were found in drought stress with explaining total 18.57% phenotypic variation and twelve pairs of them were detected in well watered condition with accounting total 38.49% phenotypic variation.
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2007年第11期926-932,共7页 Chinese Journal of Biochemistry and Molecular Biology
基金 国家重点基础研究发展规划(973计划 No.2003AA207010)项目 国家高技术研究发展计划资助项目(863计划 No.2004B17200) 洛克菲勒基金 吉首大学校人事基金资助~~
关键词 抗旱性 光合速率 叶绿素含量 QTL分析 水稻 drought resistance photosynthetic rate ( PR ) chlorophyll content ( CC ) analysis of quantitative trait loci (QTL) rice ( Oryzα sativa L. )
  • 相关文献

参考文献16

  • 1Shigesaburo Jsuonda.Photosynthetic Efficiency in Rice and Wheat.Rice Breeding[M].Manila:IRRI,1972:471-479 被引量:1
  • 2Teng S,Qian Q,Zeng D L,et al.QTL analysis of leaf photosynthetic rate and related physiological traits in rice (Oryza sativa L.)[J].Euphytica,2004,135(1):1-7 被引量:1
  • 3Kannangara C G.Part V I.The mechanism of photosynthesis in common plants,In:The Photosynthetic Apparatus[M].California:Academic Press Inc Press,1991:302-321 被引量:1
  • 4吴平,罗安程.应用分子标记研究氮素胁迫条件下水稻叶片叶绿素含量差异的遗传背景[J].Acta Genetica Sinica,1996,23(6):431-438. 被引量:34
  • 5杨权海,陆巍,胡茂龙,王春明,张荣铣,M Yano,万建民.水稻叶片叶绿素和过氧化氢含量的QTL检测及上位性分析(英文)[J].Acta Genetica Sinica,2003,30(3):245-250. 被引量:23
  • 6Wang B,Lan T,Wu W R,et al.Mapping of QTLs controlling chlorophyll content in rice[J].Acta Genet Sin,2003,30(12):1127-1132 被引量:1
  • 7Liu H Y,Zou G H,Liu G L,et al.Studies on the QTL locating of CT,LWP and SF in rice under water ladder and the correlation of them[J].Chin Sci Bull,2005,S0(2):130-139 被引量:1
  • 8Lincoln S E,Daly M J,Lander E S.Mapping Genes Controlling Quantitative Traits using Mapmaker/QTL version1.1:A Tutorial and Reference Manual,2 nd ed[M].Cambridge:A Whit Institute for Biometrieal Research Technical Report Press,1993:1-198 被引量:1
  • 9Wang D L,Zhu J,Li Z K,et al.Mapping QTLs with epistatic effects and QTL×environment interactions by mixed linear model approaches[J].Theor Appl Genet,1999,99(5):1255-1264 被引量:1
  • 10McCouch S R,Cho Y G,Yano M,et al.Report on QTL nomenclature[J].Rice Genet News l,1997,14(1):11-13 被引量:1

二级参考文献26

  • 1[1]Allard R W.Future direction in plant population genetics,evolutionand breeding.Brown A H D,Clegg M T,Kahler A L,Weir B S eds.Plant population genetics and germplam resources.Sinauer Associates Inc.:Sunderland MA,1988,1~19. 被引量:1
  • 2[2]Basten C J,Weir B S,Zeng Z B.QTL Cartographer version 1.13:A reference manual and tutorial for QTL mapping.World Wide Web URL:Http:∥statgen.ncsu.edu/qtlcart/cartographer.html,1999. 被引量:1
  • 3[3]Ben Hui Liu.Statistical genomics.CRC Press LLc,1998. 被引量:1
  • 4[4]Li Zhikang,Pinson Shannon R M,Park William D.Epistasis for three Grain Yield Components in rice (Oryza Sativa L.).Genetics,1997,145:453~465. 被引量:1
  • 5[5]McCouch S R,Cho Y G,Paul E,Blinstrub M,Morishima H,Yano M,Kinoshita T.Report on QTL nomenclature.Rice Genet Newslett,1997,14:11~13. 被引量:1
  • 6[6]Moreno-Gonzalez J.Genetic models to estimate additive and non-additive effects of marker-associated QTL using multiple regression techniques.Theor Appl Genet,1992,85:480~488. 被引量:1
  • 7[7]Patterson B D,Mackae E A,Ferguson I B.Estimation of hydrogen peroxide in higher plant.Analytical Biochemistry,1984,139:487~492. 被引量:1
  • 8[8]S Y Lin,T Sasaki,M Yano.Mapping quantitative trait loci controlling seed dormancy and heading date in rice,Oryza astiva L.,using backcross inbred lines.Theor Appl Genet,1998,96:997~1003. 被引量:1
  • 9[9]Spickett S G,Thoday J M.Regular response to selection 3.Interaction between located polygenes.Genet Res,1966,7:96~121. 被引量:1
  • 10[10]Wang D L,Zhu J,Li Z K,Paterson A H.Mapping QTLs with epistatic effects and QTL x environment interactions by mixed linear model approaches.Theor Appl Genet,1999,99:1255~1264. 被引量:1

共引文献240

同被引文献284

引证文献14

二级引证文献186

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部