摘要
目的研究胎膜早破孕妇血清中基质金属蛋白酶-9(MMP-9)和血清及羊水中肿瘤坏死因子-α(TNF-α)与绒毛膜羊膜炎的关系。方法选取胎膜早破患者67例为研究组,并选择同期正常的40例孕妇为对照组,采用酶联免疫吸附法和放射免疫法检测血清MMP-9、血清及羊水中的TNF-α水平,产后对胎膜行病理检查。结果(1)研究组羊水中TNF-α和血清中MMP-9水平明显高于对照组(P<0.05),血清中的TNF-α无明显差异(P>0.05);(2)研究组中破膜时间<24 h组血清中MMP-9水平显著低于≥24 h组(P<0.05),羊水中TNF-α差异却无统计学意义(P>0.05);(3)血清中MMP-9水平在重度和轻中度绒毛膜羊膜炎组差异显著(P<0.05),羊水中TNF-α差异无统计学意义(P>0.05)。结论测定胎膜早破孕妇血清中MMP-9和羊水中TNF-α水平可判定是否存在绒毛羊膜炎,其血清MMP-9水平的高低,还可以判定破膜时间长短和绒毛羊膜炎严重程度。
Objective To study the relation of matrix metalloproteinase-9 in serum and tumor necrosis facror-α in serum and amniotic fluid in predicting chorioamnhionitis in patients with premature rupture of membranes （PROM）. Methods The levels of MMP-9 in serum and TNF-α in serum and amniotic fluid were measured by radiolmmunoassay and ELISA in 67 cases with premature rupture of membranes as study group and 40 cases normal full-termed pregnant women as controls group. Results （1）The levels of TNF-α in amniotic fluid and MMP-9 in serum in study group were significantly higher than those in controls group（P 〈 0.05 ）, but the levels of TNF-α in serum were not（P 〉 0.05）. （2） In study group, the levels of MMP-9 of serum in 〈 24 hours group were significantly lower than those in ≥24 hours group （P 〈 0.05 ）. （3）The levels of MMP-9 in serum in serious chorioamnhionitis were significantly higher than that in diminutive chorioamnhionitis（P 〈 0.05） ,but the levels of TNF-α in amniotic fluid were not（P 〉 0.05）, Conclusions The levels of TNF-α in amniotic fluid and MMP-9 in serum were valuable clinical indices for identification of chorioamnioniris in patients with PROM. The levels of MMP-9 in serum also could assess the time of rupture of membranes and the degree of chorioamnionitis.
出处
《中国基层医药》
CAS
2007年第10期1595-1597,共3页
Chinese Journal of Primary Medicine and Pharmacy
基金
广东省汕头市重点科技计划项目(200601)
