摘要
采用水酶法提取甜杏仁油和水解蛋白。经筛选,确定选用复合植物水解酶、果胶酶12∶配比后与Alcalase蛋白酶联合作用。通过单因素试验和正交试验,确定了最佳工艺参数:固液比为15∶(W/W),复合植物水解酶与果胶酶添加总量为2.5%,反应时间5 h;碱提pH 8.5,温度60℃,碱提时间60 min;Alcalase蛋白酶反应初始pH 10,反应温度60℃,添加量1.5%,反应时间4 h。在最佳工艺条件下,游离油得率为72.58%,水解蛋白得率为82.35%。
The sugary almond oil and protein hydrolysate were produced using enzymatic hydrolysis method. Complex of cellulase,pectinase （1：2） and Alcalase showed better effect than other methods. The optimal parameters for hydrolysis were established by the single factor and orthogonal test. The optimal processing conditions were as follows： weight ratio of seeds to water 1：5（W/W）, the amount of cellulase and pectinase complex 2.5%（V/W）,reaction time 5 h;alkaline extraction pH 8.5,time 60 min,temperature 60 ℃ ;Alcalase treatment performed at pH 10,tempreture 60 ℃ with 1.5% （V/W） enzyme for 4 h. Under these conditions, the free oil and protein hydrolysate yield were 72.58% and 82.35%, respectively.
出处
《中国油脂》
CAS
CSCD
北大核心
2007年第11期26-30,共5页
China Oils and Fats
关键词
甜杏仁油
水酶法
杏仁水解蛋白
sugary almond oil
aqueous enzymatic extraction
almond protein hydrolysate
