摘要
目的:研究抵抗素(resistin)基因启动子-420C/ G多态性与日本高知地区非酒精性脂肪肝炎(Nonalcoholic steatohepatitis,NASH)的相关性.方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分别检测77例NASH患者和107例正常者resistin基因启动子-420C/G多态性的变异.酶链免疫测定法测定NASH组患者空腹12h血清resistin水平等,同时采用HOMA公式评价胰岛素抵抗指数和分泌指数HOMA- IR和HOMA-β,并用糖负荷30 min净增胰岛素/净增葡萄糖△I30/△G30比值评价早期胰岛素分泌反应及应用CT扫描评估内脏及肝脏脂肪浸润面积.结果:在校正年龄、性别等因素之后,Resistin基因启动子-420C/G的多态性分析中发现,NASH患者组的G等位基因频率与正常对照组比较,差异有显著性(0.552 vs 0.407,P<0.01).NASH患者组中GG基因型携带者的resistin血浆含量显著高于其他基因型携带者(27.1±4.7μg/L vs 16.4±6.2μg/L,P<0.001),GG基因型组肝纤维化程度更重(2.25±0.68 vs 1.61±0.65,P<0.02)且更易发生糖耐量异常(OR= 3.80,95%可信区间:1.14-12.67,P<0.05).结论:Resistin基因启动子-420位点的G等位基因与NASH发生发展具有显著相关性.
AIM： To investigate the relationship between the C/G genotypes of resistin single nucleotide polymorphism （SNP） at-420 and the development and progression of non-alcoholic steatohepatitis （NASH） in the Kochi area of Japan. METHODS： Polymerase chain reaction （PCR） and restriction fragment length polymorphism （RFLP） were used to determine a common polymorphism of the human resistin gene, -420C 〉 G, in 107 controls and 77 patients with NASH. In patients with NASH, 12-hour fasting serum resistin level was determined by enzyme linked immunosorbent assay （ELBA）. CT was used to assess liver visceral fat infiltration.tin gene were more frequent in NASH patients than in controls （0.552 vs 0.407, P 〈 0.01）. Resistin level was significantly higher in NASH patients homozygous for -420G of the resistin gene than in those with other genotypes （27.1± 4.7 μg/L vs 16.4 ± 6.2 μg/L, P 〈 0.001）. NASH patients homozygous for -420G of the resistin gene were sensitive to the progression not only of impaired glucose tolerance （OR = 3.80; 95% CI, 1.14-12.67; P 〈 0.05）, but also of liver fibrosis （2.25 ± 0.68 vs 1.61 ± 0.65, P 〈 0.02）. CONCLUSION： The G homozygote of the resistin gene SNP at -420 is associated with NASH in the Kochi area of Japan.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第30期3204-3209,共6页
World Chinese Journal of Digestology
关键词
抵抗素
单核苷酸多态性
胰岛素抵抗
非酒精性脂肪肝炎
聚合酶链反应-限制性片断长度多态性
Resistin
Single nudeotide polymorphism
Insulin resistance
Nonalcoholic steatohepatitis
Polymerase chain reaction-restriction fragmentlencjbh polymorphism
