摘要
目的:通过观察高压氧处理后人鼻咽癌细胞系CNE2Z细胞MMP-9和VEGF的表达,探讨高压氧对鼻咽癌细胞浸润与转移的影响及其机制。方法:将实验用人鼻咽癌CNE2Z细胞分为对照组(A组)、乏氧组(B组)、乏氧加高压氧组(C组)、单纯高压氧组(D组)四组。各组分别处理后,采用细胞免疫化学染色法检测各组鼻咽癌细胞MMP-9、VEGF的表达水平,并在显微镜下观察各组MMP-9、VEGF的表达情况。结果:鼻咽癌CNE2Z细胞MMP-9、VEGF表达的平均光密度值A组与B组比较差异有显著性意义(P<0.05);C组与B组比较差异有显著性意义(P<0.05);D组平均光密度值比B组明显降低,但D组与B组比较差异无显著性意义(P>0.05);A、C和D三组之间比较差异无显著性意义(P>0.05)。结论:高压氧处理可使乏氧条件下人鼻咽癌CNE2Z细胞MMP-9、VEGF高表达减少,而对正常条件下鼻咽癌CNE2Z细胞MMP-9、VEGF的表达无影响,从而提示高压氧处理可能是通过改善乏氧状态而抑制鼻咽癌细胞的浸润与转移。
Objective： To investigate the effect and the possible influential mechanisms of hyperbaric oxygen （HBO） on the infiltration and metastasis in human nasopharyngeal carcinoma （NPC） cell line CNE2Z by observing MMP-9 and VEGF expressions of nasopharyngeal carcinoma cells after HBO treatment. Method： Nasopharyngeal carcinoma （NPC） cells CNE2Z were divided into 4 groups randomly： Group A ： control group; Group B： oxygen deficiency; Group C： oxygen deficiency plus hyperbaric oxygen; Group D： hyperbaric oxygen. The MMP-9 and VEGF expressions in CNE2Z cells of all groups were detected by SP immunocytochemical staining and the expressed images were observed under microscope, Result：Average optical density（OD） of MMP-9 and VEGF expressions were statistically significant different between group A and group B （P〈0.05）, and between group C and group B also. Average optical of group D were down-regulated compared with group B, but there was no statistically significant difference （P〉0.05）. No significant difference was found between group A, group C and group D on the average OD （P〉0.05）. Conclusion： HBO treatment could decrease the MMP-9 and VEGF high-expressions in human NPC cell line CNE2Z in anaerobic condition, and it did not affect the MMP-9 and VEGF expressions in CNE2Z cells in normal condition. It showed that HBO treatment could inhibit the infiltration and metastasis of NPC cells by improving the oxygen deficiency condition.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2007年第11期998-1001,I0002,共5页
Chinese Journal of Rehabilitation Medicine
关键词
鼻咽癌
CNE2Z细胞株
高压氧
基质金属蛋白酶-9
血管内皮生长因子
nasopharyngeal carcinoma
CNE2Z cell line
hyperbaric oxygen
matrix metallo-proteinase 9
vascular endothelial growth factor
