摘要
目的:观察APE1 siRNA表达载体pSilence APE1基因放射治疗对骨肉瘤裸鼠移植瘤生长的抑制作用。方法:人骨肉瘤细胞9901荷瘤裸鼠30只随机分为3组:对照组、单独放射治疗组和pSilence APE1+放疗联合治疗组。实验治疗第15天处死动物,绘制肿瘤生长曲线,免疫组化SP法检测骨肉瘤细胞APE1蛋白的表达、骨肉瘤细胞的微血管密度和TUNEL法检测肿瘤细胞凋亡的情况。结果:pSilence APE1+放疗联合治疗组和对照组、单独放射治疗组的肿瘤大小存在显著性差异(P<0.05)。pSilence APE1+放疗联合治疗组肿瘤组织APE1表达显著降低;pSilence APE1+放疗联合治疗组肿瘤微血管密度明显低于对照组和单独放射治疗组(P<0.01),而肿瘤细胞凋亡显著增加(P<0.01)。结论:实验结果表明,pSilence APE1基因放射治疗能显著抑制骨肉瘤的生长。
Objective： To observe the inhibitory effect of APE1 siRNA generated by vector pSilence in conjunction with local radiotherapy on osteosarcoma cells of nude tumor-bearing mice. Methods： Thirty nude mice inoculated with 9901 osteosarcoma cells were randomly divided into 3 groups： the control group, the radiotherapy group and the group that received pSilence APE1 therapy plus radiotherapy. On the 15th day of the experimental treatment, the mice were sacrificed and the tumor growth curve was plotted. Meanwhile, the expression of APE1 protein and intratumor microvessel density （MVD） were observed by immunohistochemistry. The apoptotic index was detected by the Terminal dUTP nick end labeling （TUNEL） technique. Results： There was a significant difference in tumor volume among the group of pSilence APE1 plus simple radiotherapy, the control group and the radiotherapy group （P〈0.05）. The APE1 expression in the tumor cells in the group of pSilence APE1 plus simple radiotherapy was significantly decreased. The intratumoral microvessel density （MVD） in the group of pSilence APE1 plus simple radiotherapy was significantly lower compared to the control group and the radiotherapy group （P〈0.01）, while the apoptosis index was much higher （P〈0.01）. Conclusion： Using pSilence APE1 in conjunction with radiotherapy can significantly inhibit the growth of osteosarcoma.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2007年第4期230-233,共4页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金(编号:30472004)
重庆市自然科学基金项目资助(编号:2004BB5246)
