摘要
本研究在已构建的副结核分枝杆菌Ｃ２株ＤＮＡ基因文库的基础上，应用正、反向杂交试验从基因文库中筛选出特异性片段ＰＴＰ３１，以光敏生物素标记制成ＤＮＡ探针。用此ＤＮＡ探针以及粪检菌和ＥＬＩＳＡ三种方法分别对３２份副结核菌素（ＰＰＤ）变态反应阳性牛粪便及相应血清样品进行检测，其检出率分别为４７％（１５／３２）、５６％（１８／３２）和３４％（１１／３２）。对随机采集的２７６份牛粪便及血清样品，３种方法的检出率分别为１０％（２７／２７６）、１３％（３６／２７６）和７％（７９／２７６）。本研究结果表明，ＤＮＡ探针与粪检菌呈现正相关性，ＤＮＡ探针比ＥＬＩＳＡ方法能够检出更多的阳性数。
The mycobacterium paratuberculosis were detected by three different diagnostic methods:DNAprobe,fecal culture and ELISA 32 samples of fecales and sera from the cattle,which are positive in allergic test for M Paratuberculosis PPD,were identified with the three methods The positive rate is 47%(15/32),56%(18/32) and 34% respectively The conformity rate between DNA probe and fecal culture was 66% 276 samples selected at random were detected by these methods The positive rate was 10%(27/276),13%(36/276)and 7%(19/276)respectively These results suggest that there is direct relationship between DNA probe method and fecal culture method and that DNA probe method is more sensitive than ELISA
