摘要
为探索副结核特异性蛋白MAP0862与MAP1345在牛副结核病血清学诊断中的作用,将通过串联表达获得的融合蛋白MAP0862-1345纯化定量后包被酶标板,经过对反应条件的优化,初步建立了基于融合蛋白MAP0862-1345的间接ELISA诊断方法。使用建立的ELISA方法对牛副结核病阳性血清、牛结核病阳性血清、牛布病阳性血清、卡介苗免疫牛血清、健康牛血清检测的结果表明其具有较好的特异性;使用该方法与IDEXX副结核病抗体检测试剂盒共同对300份临床血清样本检测,总符合率为92.7%。
To explore the effect of MAP0862 and MAP1345 in the diagnosis of Bovine paratuberculosis,the specific protein acquired by tandem expression was used as coating antigen after purified and quantification,an indirect ELISA was preliminary established after a series of optimization. The specificity of this method was verified by MAP antisera,Brucella antisera,Bovine tuberculosis（ TB） antisera,BCG antisera and negative sera. The detection result of 300 sera showed that the coincidence rate of MAP0862- 1345 indirect ELISA with commercial kit was 92. 7%.
出处
《中国兽药杂志》
北大核心
2016年第6期16-21,共6页
Chinese Journal of Veterinary Drug
基金
北京市科技新星计划(xx2013099)
现代农业人才支撑计划项目
关键词
副结核分枝杆菌
串联表达
间接ELISA
Mycobacterium avium subsp
paratuberculosis
tandem expression
indirect ELISA
