摘要
应用1对乙型肝炎病毒（HBV）S基因保守区的引物，采用PCR方法从屠宰猪肝、血清中检测到了HBV，序列分析表明，扩增片段与已发表的HBVS基因的同源性高达98％～100％。电镜负染色样品观察结果表明，在HBV表面抗原ELISA检测强阳性反应的血清样品中存在有形态、大小与人HBV Dane颗粒和小球状颗粒相似的病毒粒子。针对戊型肝炎病毒（HEV）（）RF2／ORF3重叠区设计了简并引物，采用巢式RT-PCR对屠宰猪肝和血清样品进行了检测。结果表明，部分屠宰猪肝中存在HEV。
The study reported the presence of swine hepatitis B（HBV） and E（HEV） viruses in the liver and serum in pigs in China. Two primers for S gene of swine HBV were designed and synthesized according to sequences of the S genes of human HBV in the literature. Swine liver and serum samples were collected from a slaughter house. Swine S genes were amplified with RT-PCR and sequenced. Results showed that sequences of S genes of swine and human HBV viruses shared 98%--100% homology. Emission transmission electron microscopy examination of HBV-positive serum revealed presence of virus particles 40--20 nm in diameter. These particles were similar to human HBV particles in terms of both diameters and shapes. A positive serum was defined by the presence of the HBV surface antigen using the ELISA method. Responses in a nested RT-PCR assay to a pair of degenerated primers designed from ORF2/ORF3 overlapping region indicated presence of gene sequence of HEV in the liver,but not serum,in some pigs.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2007年第11期921-925,共5页
Chinese Veterinary Science
基金
国家自然科学基金项目(30771588)
关键词
猪
乙型肝炎病毒
戊型肝炎病毒
电镜观察
swine
hepatitis B virus
hepatitis E virus
electron microscopy
