摘要
目的:探讨leptin在急性炎症反应中的作用.方法:采集正常大鼠下丘脑、肺、肝、脾、胃、十二指肠、肾、附睾脂肪垫、睾丸等重要脏器标本,以RT-PCR法检测leptinmRNA表达的组织分布;并建立大鼠盲肠结扎穿孔模型,设立假手术组(A)和脂肪乳组(B)、单纯损伤组(C)、雌二醇组(D)、胰岛素组(E)等实验组,采用RT-PCR检测脂肪、肝及肺内leptinmRNA表达的变化.结果:正常大鼠的上述9种重要脏器内均有leptinmRNA表达,肾脏内含量最高而睾丸内含量最低.大鼠盲肠结扎穿孔12h后,与A组leptinmRNA表达水平相比,其在B组脂肪内表达显著增高而在肝、肺内表达显著降低,在C组肝内表达无显著差异而在脂肪、肺内表达显著降低,在D组肺内表达显著增高而在脂肪、肝内表达显著降低,在E组肺内表达无显著差异而在脂肪、肝内表达显著降低.脂肪乳对leptinmRNA表达的影响具有中枢分泌组织(脂肪)内诱导而外周脏器内抑制的双向模式.结论:LeptinmRNA表达水平在干预急性肠道损伤后能量代谢和神经-内分泌功能时发生敏感变化,提示leptin可能作为一种核心保护因子促进内环境的稳定.
AIM： To explore the acute inflammation. role that leptin plays in METHODS： Vital organ samples, including hypothalamus, lung, liver, spleen, stomach, duodenum, kidney, epididymal fat pad and testis, were collected from normal rats, and the levels of leptin mRNA expression in those samples were determined by reverse transcriptasepolymerase chain reaction （PCR）. A cecal ligation and perforation model was established in rats. The rats were divided into sham-operation（A）, intralipid injection （B）, injury （C）, estradiol injection （D） and insulin injection （E） groups to assess energy metabolism and neuroendocrine function. Reverse transcriptase-PCR was also used to detect leptin mRNA expression in adipose tissue, liver and lung. RESULTS： Leptin mRNA expressions were confirmed in all nine vital organs examined, with the highest seen in kidney and the lowest levels seen in testis. By twelve hours after cecal ligation and perforation, compared with the level of leptin mRNA expression in the sham-operation group, that in the intralipid injection group was increased significantly in adipose tissue but decreased significantly in liver and lung; that in the injury group showed no difference in liver but was decreased significantly in adipose tissue and lung; that in the estradiol group was increased significantly in lung but decreased significantly in adipose tissue and liver; and that in the insulin group showed no difference in lung but was decreased significantly in adipose tissue and liver. The effect of intralipid injection on leptin mRNA expression was found to be a dual-direction pattern, as intralipid injection induced leptin mRNA expression in central secretory tissue （adipose tissue）, but inhibited it in peripheral organs. CONCLUSION： Leptin mRNA expression shows a sensitive change when intervening energy metabolism and neuroendocrine function after acute intestinal injury, which suggests that leptin may be a key protective factor promoting homeostasis.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第28期2995-3000,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30670821
30700868
解放军总医院苗圃基金资助项目
No.06MP83~~
