摘要
目的探讨缺血预处理(IP)对不同冷保存时间大鼠移植肠的保护作用。方法将80只SD大鼠随机分为2组:即非预处理组(0-L组)与预处理组(L组),每组分别再分为4小组,按冷缺血时间分为3(L3)、6(L6)、12(L12)及18(L18)组,每组10只。建立大鼠缺血预处理模型,将各组供肠冷保存相对应时间。供肠行原位移植后再灌注1h取材,对其进行组织学观察,并观测NF-κB的表达。结果相同冷缺血时间的预处理组损伤明显较非预处理组轻,表现为预处理组较非预处理组小肠绒毛排列整齐,肌层水肿较轻,Chiu氏评分升高。预处理组较非预处理组上皮细胞NF-κB的表达减弱(P<0.05)。结论缺血预处理对不同冷保存时间移植肠具有保护作用。
Objective To evaluate the protective effect of ischemic preconditioning against cold ischemia and reperfusion injury of rat intestinal graft following orthotopic transplantation. Methods Eighty SD rats were randomly assigned into two groups with and without ischemic preconditioning, and each group was divided into 4 subgroups （n=10） according to the intestinal graft cold ischemia time of 3, 6, 12, and 18 h, respectively. Ischemic preconditioning model was established, and the small intestinal graft was preserved at 4 ℃ in Ringer lactate solution for the corresponding time, followed by orthotopic transplantation of the graft. The graft samples were collected for histological examination 1 h after reperfusion, and nuclear factor-κB （NF-κB） expression in the epithelial cells was detected. Results Ischemia preconditioning obviously relieved the histological ischemia/reperfusion injury, as shown by regular alignment of the small intestinal villi, alleviated muscular layer edema and decreased expression of NF-κB in the epithelia of the graft in groups with cold preservation. Conclusion Ischemic preconditioning can protect the intestinal graft from cold ischemia/reperfusion injury, and NF-κB is an important cytokine in ischemia preconditioning.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第11期1764-1766,共3页
Journal of Southern Medical University
关键词
小肠移植
冷保存
缺血再灌注损伤
缺血预处理
NF-κB
small bowel transplantation
cold preservation time
ischemia-reperfusion injury, ischemia preconditioning
nuclear factor-κB
