摘要
目的:观察奥沙利铂慢性神经毒性对大鼠背根神经节细胞尼氏体及P物质的影响。方法:30只Wistar大鼠随机分成奥沙利铂组、对照组各15只。奥沙利铂组腹腔注射奥沙利铂4mg/kg,对照组腹腔注射等容积5%葡萄糖注射液,每周2次,共9次。每次给药后2h检测50%缩足阈,最后一次给药后24h取第5腰椎背根神经节切片、染色。观察背根神经节神经元细胞形态以及尼氏体和P物质的形态和积分光密度。结果:奥沙利铂组大鼠50%缩足阈从第3次给药后明显低于对照组(P<0.01);背根神经节内神经元细胞体、细胞核及核仁面积减小(P<0.05,P<0.01),偏心核与多核仁比例增高(P<0.01);尼氏体、P物质积分光密度较对照组降低(P<0.05,P<0.01)。结论:奥沙利铂引起背根神经节尼氏体及P物质的改变,与周围神经病变有关。
Objective：To observe the influence of chronic oxaliplatin neumtoxicity on Nissl bodies and substance P in dorsal root ganglion in rats. Methods： Thirty Wistar rats were randomly divided into two groups： oxaliplatin group and control group. The rats in the oxaliplatin group were given oxaliplatin 4 mg/kg twice weekly by intraperitoneal injection for 9 times altogether. The rats in the control group were given the same volume of 5% glucose. 50% paw withdrawal threshold （50% PWT） was measured 2 hours after each injection. The L5 dorsal root ganglion was sliced and stained 24 hours after the last injection. The morphology of dorsal root ganglion neurons, and the morphology and integrating optical density of Nissl bodies and substance P were observed. Results： 50% PWT of rats in the oxaliplatin group decreased significantly after the third injection than that in the control group （ P 〈 0. 01 ）. Neuropathological examination showed, in comparison to the control group, the area of cyton, nucleus, and nucleolus in dorsal root ganglion diminished （ P 〈 0. 05, P 〈 0. 01 ）, the proportions of decentered nucleus and polynucleolus increased （ P 〈 0.01 ）, the integrating optical density of Nissl bodies and substance P decreased （ P 〈 0. 05, P 〈 0. 01 ）. Conclusion： The peripheral neuropathy caused by oxaliplatin is related to the changes in Nissl bodies and substance P in dorsal root ganglion neuron.
出处
《药物不良反应杂志》
2007年第5期320-323,共4页
Adverse Drug Reactions Journal
关键词
奥沙利铂
神经毒性
背根神经节
尼氏体
P物质
oxaliplatin
neurotoxicity
dorsal root ganglion
Nissl body
substance P
