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不同外源激素和培养温度对马铃薯试管薯形成的影响 被引量:10

Effects of Exogenous Hormones and Temperature on the Formation of Potato Micro-tuber in Vitro
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摘要 以云南栽培的3个马铃薯品种(合作88、大西洋以及转心乌)的脱毒试管苗为材料,研究了不同温度以及不同的外源激素(6-BA,CCC)对试管薯形成的影响。结果表明:以MS+8%蔗糖为基本培养基,在全黑暗条件下,加入CCC比加入BA更有利于诱导结薯。大西洋最适宜的诱导试管苗结薯的培养组合为:CCC 300 mg/L+20℃;合作88为:CCC 500 mg/L+20℃;转心乌则为:CCC 300 mg/L+25℃。 Virus -free plantlets of three potato cuhivars (Solanum tubersum L. ) ( Cooperation - 88, Atlantic, Zhuanxinwu) in Yunnan Province were used as the experimental materials. The effects of different combinations of temperature and exogenous hormone(6 -BA and CCC) on the formation of micro-tubers were studied. The results indicated that: the different varieties potatoes required different temperatures and concentration of exogenous hormone. CCC indicated better micro-tubers than BA in full darkness. The optional combinations for Atlantic, Cooperation -88 and Zhuanxinwu were CCC 300 mg/L + 20 ℃, CCC 500 mg/L + 20 ℃ and CCC 300 mg/L + 25 ℃, respectively.
出处 《云南农业大学学报》 CAS CSCD 2007年第6期824-828,共5页 Journal of Yunnan Agricultural University
基金 云南省科技厅"十一五"攻关项目资助(2006NG08)
关键词 马铃薯 试管薯 矮壮素 6-苄氨基嘌呤 温度 块茎诱导 potato micro-tuber CCC 6 - BA temperature micro-tuber inducement
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