摘要
研究了Cu-酪氨酸荧光静态猝灭体系,络合物为Cu（Tyr）2^2＋,猝灭常数K为1.59×1010。以时间驱动模式代替浓度模式,在pH 8.8的硼砂介质中,测得Cu2＋的线性范围为4.00×10^-7～1.12×10^-5mol/L和8.00×10^-6～1.12×10^-4mol/L,相关系数R=0.9996/0.9992,检出限（3σ）为6.6×10^-8mol/L,相对标准偏差（RSD）为0.78%（n=11,C（Cu^2＋）=2.4×10^-6mol/L）。本方法可用于实际样品中痕量铜的测定。
A new fluorescence method has been developed for the determination of copper（ Ⅱ ）. It was based on the Cu^2＋ -tyrosine static fluorescence quenching system, which quenching constant was 1.59 × 10^10 L^2· mol^-2, as formed Cu（Tyr）2^2＋ . In the borax buffer solution of pH 8.8, substituted TimeDrive mode for concentration mode, the linear range for Cu^2＋ concentration was from 4.00×10^-7 to 1.12 × 10^-4 mol/L （correlation coefficient, 0.9996） with a de- tection limit （3or） of 6.6 × 10^-8 mol/L, and the precision of the method was 0.78% （RSD） for eleven replicates of a standard （ c（Cu^2＋ ） = 2.40 × 10^-6 mol/L）. Due to simplicity, high sensitivity and precision, the proposed method has been used directly to determine the concentration of Cu^2＋ in water and hair.
出处
《分析试验室》
CAS
CSCD
北大核心
2007年第11期70-73,共4页
Chinese Journal of Analysis Laboratory
关键词
时间驱动
荧光猝灭
酪氨酸
铜离子
Timedrive
Fluorescence quenching
Tyrosine
Copper（Ⅱ）
