摘要
采用荧光光谱(三维荧光、时间驱动模式)和紫外一可见吸收光谱研究了芦丁(Rutin)与牛血清白蛋白(BSA)间的相互作用,讨论了药物对BSA构象的影响,证实了二者间相互作用为单一的动态猝灭过程,求出了猝灭常数,并依据能量转移理论确定了药物与蛋白质相互作用的最近距离。
The reaction between Rutin and bovine serum albumin （BSA） was studied using 3D fluorescence spectra and ultra-violate spectra. The conformational change of BSA due to the addition of extract was discussed. It was proved that only dynamic quenching exited between Ratin and BSA. Quenching constant was obtained and the distance between Rutin and BSA was determined according to the theory of energy transfer.
出处
《黄山学院学报》
2008年第5期53-56,共4页
Journal of Huangshan University
基金
黄山学院科研基金资助(2007xkq014)
