摘要
目的构建野生小鼠抗肺肿瘤基因差异表达文库。方法应用抑制性消减杂交(SSH)技术对TAF1小鼠与A/wy近交系小鼠中差异表达的抗肺肿瘤基因进行筛选,构建消减cDNA文库,并对部分克隆进行核苷酸序列分析,利用BLAST检索分析其可能代表的基因。结果166个差异表达克隆测序后进行BLAST分析,其中134个克隆与87个已知小鼠的mRNA或cDNA序列同源,32个克隆与14个DNA序列同源;87个已知基因按功能分为7大类。其他32个克隆中,20个克隆与9个鼠DNA序列同源,其中可能含有新基因,2个克隆与1个细菌序列同源,3个克隆为克隆载体。结论应用SSH技术成功构建了野生小鼠抗肺肿瘤基因差异表达文库。
Objective To construct the anti-lung tumor gene differentially expressed bank of wild mouse and to explore the mechanisms of the TW wild mouse suppressing the occurring of lung tumor. Methods Using suppression subtractive hybridization （SSH） technique, the differentially expressed genes between TAF1 mouse and A/wy mouse were selected out and the subtracted cDNA bank was constructed.166 clones were performed DNA sequencing and then were assayed by blast programme. Results Among the blast results of 166 differentially expressed clones, 87 known genes （mRNA or cDNA） were in homology with 134 clones and were divided into 7 classifications according to the biological role.14 DNA fragments were in homology with 32 clones,in which 20 clones were in homology with 9 mouse DNA sequences, 2 clones were in homology with one bacterial gene sequences and 3 clones were clone vector. Conclusion With SSH technique, the anti-lung tumor gene differentially expressed bank of wild mouse are successfully constructed.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2007年第10期594-599,共6页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
天津市科委重点基金项目(003803511)
关键词
肺肿瘤
基因
差异文库
小鼠
Lung neoplasmas
Genes
Gene library
Mice
