摘要
用ＢＡＬＢ／ｃ×ＬＡＣＡＦ＿１和ＢＡＬＢ／ｃ×Ｃ＿（５７）Ｆ＿１杂交小鼠与纯系ＢＡＬＢ／ｃ小鼠生产腹水制备单克隆抗体，观察各种小鼠对杂交瘤细胞的耐受性，产生腹水的能力及腹水中蛋白质含量和抗体效价。ＢＡＬＢ／ｃ×ＬＡＣＡＦ＿１鼠的平均腹水产量，雄鼠为ＢＡＬＢ／ｃ鼠的２～３倍，雌鼠为１．４倍左右。对杂交瘤细胞的耐受性，腹水中蛋白质含量及抗体效价与ＢＡＬＢ／ｃ小鼠比较均无显著差异。ＢＡＬＢ／ｃ×Ｃ＿（５７）Ｆ＿１小鼠的腹水产量明显低于ＢＡＬＢ／ｃ小鼠，而且死亡率较高。ＢＡＬＢ／ｃ×ＬＡＣＡＦ＿１小鼠生长快，易于饲养繁殖，价格便宜，腹水产量高，是一种替代ＢＡＬＢ／ｃ小鼠生产单克隆抗体腹水较理想的鼠种。
The ability of producing ascitic monoclonal antibodies of the first generation hybridmice,BALB / c × LACA and BALB / c × C_(57),were compared with that of BALB / c parents.Ascitic fluids produced in the hybrid F_1 and BALB / c mice were evaluated in terms of volume,antibody titers and protein levels.The results indicated that BALB / c × LACA F_1 producedsignificantly more ascitic fluid of equally high antibody titers and protein levels compared to theBALB / c parent, approximately 1.4 times more for the females and 2-3 times more for the males.BALB/c×LACA F_1 grow faster and cost less than the BALB/c.It is suggested that BALB/c×LACA F_1 mice may be used in production of ascitic monoclonal antibodies instead of BALB/c mice.
出处
《军事医学科学院院刊》
CSCD
北大核心
1995年第3期175-179,共5页
Bulletin of the Academy of Military Medical Sciences
