摘要
目的：调查唐山地区胃癌组织中EBV的感染状况,以分析EB病毒相关胃癌（GC）的临床流行病学特征,以及分析EB病毒在不同年龄以及不同组织学类型胃癌形成过程中的作用。方法：选择胃癌患者手术切除后经石蜡包埋的组织标本共202例。标本经切片和常规脱蜡处理后提取DNA,首先经PCR进行β-actin DNA检测,阳性标本用PCR法检测EBNA1（EB病毒核抗原1）DNA。结果：202份标本β-actin DNA阳性166例,阳性率为82%;166例标本中EBNA1PCR阳性例数为14例,阳性率为8.4%;166例β-actin DNA阳性的胃癌标本中,腺癌150例,经EBNA1PCR检测EB病毒DNA阳性例数在腺癌为14,阳性率为9.3%;150例胃腺癌中,≤45岁15例,46~65岁93例,≥66岁42例,经EBNA1PCR检测EB病毒DNA阳性例数分别为1、9、4例,阳性率分别为6.7%、9.7%、9.5%,差异无统计学意义。结论：EB病毒感染胃癌形成过程中所起的作用与患者年龄无关,而且与癌组织类型无关。
Objective：To determine the occurring frequency, and to clarify the clinic epidemiological characteristics, and to analyze the roles of EBV in the diffusion and metastasis of EBV-related gastric carcinoma in Tangshan area, we investigated the situation of EBV infection in the gastric carcinoma with different histological classification and different age in Tangshan Area. Methods： 202 cases of formalin-fixed and paraffin-embedded tissue coming from surgically resected gastric carcinomas in Tangshan area, were confirmed further by histopathological observation by hematoxylin-eosin （HE） staining. The DNAs were extracted according to the method described by Van Beek with some modifications, and were screened for β-actin DNA detection. EBV infection was analyzed by detecting EBNA1 DNA by PCR. Results：EBV DNA were detected in 14 of 166 β-actin DNA positive cases （8.4%）. 14 positive cases came farom 150 adenocarcinomas （9.3%）. Conclusion：The occurring rate of EBV-related gastric carcinoma was 8.4% in TangShan area, which suggests that EBV infection is likely to be involved in the occurrence or development of gastric carcinoma. The infectious rate showed no statistics auy significant differences between different age group and different histological classification.
出处
《陕西医学杂志》
CAS
北大核心
2007年第7期871-872,881,共3页
Shaanxi Medical Journal
基金
教育部留学回国人员科研启动基金(教外司留[2000]479)
