摘要
目的检测EB病毒相关性胃癌(EBVaGC)的阳性率及分析其临床病理特征,探讨EBVaGC微血管生成、微淋巴管生成的可能机制。方法利用原位分子杂交技术检测EBV编码小RNA1,从600例胃癌组织标本中,筛选出EB病毒相关性胃癌组织标本,对其采用免疫组化方法检测LMP1、BHRF1、VEGF-C、LYVE-1和CD34的表达水平,分析其可能存在的关系。结果在600例胃癌标本中EBVaGC的阳性率为5.0%,临床病理因素分析发现EBVaGC与性别、病理类型、发生部位及淋巴结转移有关(P<0.05),而与年龄无关(P>0.05)。在30例EBVaGC标本中LMP1呈低表达,与性别、分期及周围淋巴结转移无关(P>0.05),而BHRF1、VEGF-C高表达,均与TNM分期和周围淋巴结转移有明显的关系(P<0.05);EBVaGC中MVD与性别及周围淋巴结转移无关,而与TNM分期显著有关(P<0.05);MLVD与TNM分期和周围淋巴结转移密切相关(P<0.05)。BHRF1的表达与MVD无显著关系,而与MLVD有明显差异(P<0.05)。VEGF-C阳性表达的19例EBVaGC组织中,MVD和MLVD均明显高于阴性组,两组比较其差异具有显著的统计学意义(P<0.05)。结论 EBVaGC与性别、病理类型、发生部位及淋巴结转移有关,而与年龄无关。EBVaGC中LMP1的表达率低,BHRF1的表达率高,可能与EBVaGC较少发生淋巴结转移有关。VEGF-C可能参与EBVaGC的微血管和微淋巴管生成,其高表达间接促进肿瘤细胞沿新生的淋巴管迁徙和转移。
Objective To detect the positive rate of Epstein-Barr virus-associated gastric carcinoma（EBVaGC） in gastric carcinoma and analyze the clinical and pathological features, and explore the possible mechanisms with the process of angiogenesis and lymphangiongenesis in patients with EBVaGC. Methods We selected 30 cases of EBVaGC tissue from 600 cases of gastric cancer tissue by in situ hybridization of small RNA1 EBV-encoded, and detected the expression of LMP1, BHRF1, VEGF-C, LYVE-1 and CD34 in EBVaGC tissue specimens using immunohistochemical staining and then analyzed the correlations between them. Results The positive rate of EBVaGC in600 cases of gastric cancer specimens was 5.0%, the analysis of clinical and pathological factors found EBVaGC had relations with gender,histological type, location and lymph node metastasis（P 0.05）, but not related to age（P 0.05）. The expression of LMP1 was low in 30 cases, and had no relations with gender, staging and lymph node metastasis. However, the expressions of BHRF1 and VEGF-C were high, and had obvious relations with TNM staging and lymph node metastasis（P 0.05）. MVD in EBVaGC had no relations with gender and lymph node metastasis, but had obvious relations with TNM staging（P 0.05）. MLVD was closely related to TNM staging and lymph node metastasis（P 0.05）. The expression of BHRF1 was not related to MVD, but was closely related to MLVD（P 0.05）. VEGF-C positive expression in 19 cases of EBVaGC tissue, expressions of MVD and MLVD were both higher than that of negative group, and there were statistical difference between two groups（P 0.05）. Conclusions EBVaGC has relations with gender, histological type, location and lymph node metastasis, but not with age. Low expression of LMP1 and high expression of BHRF1 in EBVaGC which may be related to less lymph node metastasis in EBVaGC occurred. Over-expression of VEGF-C demonstrates that VEGF-C may be involved in angiogenesis and lymphangio-genesis of EBVaGC, which indirectly promotes tumor cell＇s mi
出处
《临床医学工程》
2014年第5期558-561,共4页
Clinical Medicine & Engineering
基金
山东省科技发展计划项目(项目编号:2010GW20237)
关键词
EB病毒相关胃癌
血管内皮生长因子-C
微血管生成
微淋巴管生成
Epstein-Barr virus associated gastric carcinoma
Vascular endothelial growth factor C（VEGF-C）
Neovasculariza-tion
Lymphangiogenesis
