摘要
目的观察总热量正常、不同含量蛋白质饮食长期饲养对正常大鼠葡萄糖刺激的胰岛素分泌的影响。方法选择8周龄健康雄性Wistar大鼠51只,分普通饲养组(NC,n=16)、高蛋白饲养组(HP,n=19)、低蛋白饲养组(LP,n=16)3组,各组大鼠日摄入总热量相同,共饲养24周。饲养期间,观察大鼠体重、空腹血糖(FBG)、空腹胰养岛素(FINS)水平的变化。于32周龄时,行静脉葡萄糖耐量试验(IVGTT),观察各组大鼠葡萄糖刺激的胰岛素分泌,尤其是胰岛素分泌第一时相的差异。结果与NC组比较,HP组及LP组的体重和内脏脂肪重量I、VGTT各点血糖均无显著差异,HP组5 min、10 min血清胰岛素水平显著升高[(6.5±0.8)ng/mL vs(4.9±0.7)ng/mL,P<0.001;(3.9±0.6)ng/mL vs(3.1±0.5)ng/mL,P<0.001],而LP组无显著差异。结论长期高蛋白饲养大鼠第一时相胰岛素分泌显著升高,低蛋白饲养大鼠胰岛素分泌则无明显改变。
Objective To investigate the effects of a long-term protein diet with normal total calorie intake on glucose-stimulated insulin secretion in normal rats. Methods 51 healthy male Wistar rats （8 weeks old） were selected and divided into three groups： the normal food group （NC, n = 16）, the high protein diet group （HP, n = 19） and the low protein diet group （LP, n = 16）. The total calorie ingestion of each rat per day was similar and was maintained for 24 weeks. Body weight, fasting blood glucose （FBG） and fasting plasma insulin （FINS） were monitored during feedings. The insulin secretary functions of β cells, especially the acute insulin response（AIR） were determined by the intravenous glucose tolerance test （IVGTT） in 32-week-old rats. Results 1. Body weight and visceral fat mass： Compared with the NC group, there were no differences of the HP or LP groups. 2. IVGTT： （ 1 ） Blood glucose： There were no differences among the three groups. （2） Plasma insulin： 5 min- and 10 min- plasma insulin levels were markedly higher in the HP group than in the NC group [ （6.5±0.8） ng/mL vs （4.9 ±0.7 ） ng/mL, P 〈 0.001 ; （3.9 ± 0.6） ng/mL vs （3.1 ± 0.5 ） ng/mL, P 〈 0：001 ], and there were no differences between the LP and NC groups. Conclusion Long-term high protein ingestion with total calories may significantly enhance the AIR by IVGTT in rats, While low protein intake has no significant effect on insulin secretion in rats.
出处
《山东大学学报（医学版）》
CAS
北大核心
2007年第10期977-980,共4页
Journal of Shandong University:Health Sciences
基金
山东省自然科学基金重点项目(Z2006C09)
关键词
饮食蛋白
血糖
胰岛素
胰岛细胞功能
Dietary protein
Blood glucose
Insulin
Islet cell function
