摘要
目的：研究抗角蛋白自身抗体（ＡＫａｕｔｏＡｂ）对白细胞介素１β（ＩＬ－１β）诱发角朊细胞增殖的影响．方法：采用角朊细胞无血清培养方法，以３Ｈ－ＴｄＲ标示角朊细胞增殖程度，检测ＩＬ－１β刺激角朊细胞及ＡＫａｕ－ｔｏＡｂ处理角朊细胞的增殖代谢．结果：１６００Ｕ／ｍｌ及以上浓度ＩＬ－１β对角朊细胞增殖有明显促进作用（Ｐ＜０．０１），正常血清浓度ＡＫａｕｔｏＡｂ对ＩＬ－１β引起的角朊细胞增殖有抑制作用（Ｐ＜０．０１）．结论：ＡＫａｕｔｏＡｂ抑制ＩＬ－１β引起的角朊细胞增殖，提示皮肤炎症反应中，角朊细胞内沉积的ＡＫａｕｔｏＡｂ对角朊细胞的过度增殖有调节作用．
Objective: The present paper, one part of systematical studies of antikeratin auto antibody (AK auto Ab), was aimed at investigating the effect of AK auto Ab on the proliferation of keratinocytes induced by inflammatory factor interleukin 1β (IL 1β). Methods: Serum free keratinocyte culture was used, and the proliferation degree of keratinocytes was demonstrated by the thymidine incorporation into keratinocytes. Results: 1600 U/ml and higher concentrations IL 1β could significantly increase the prrliferation of cultured keratinocytes ( P <0.01), and normal sera concentration AK auto Ab could obviously inhibit the proliferation of cultured keratinocytes induced by IL 1β ( P <0.01). Conclusion: In the skin inflammatory reaction, AK auto Ab deposited in keratinocytes may regulate the hyperproliferation of keratinocytes, the present study also contributes basic theory to the application of AK auto Ab in clinical treatment.
出处
《第四军医大学学报》
1997年第2期162-164,共3页
Journal of the Fourth Military Medical University
关键词
自身抗体
角蛋白
角朊细胞
白细胞介素
细胞培养
autoantibody, keratin keratinocytes interleukin 1 proliferation, keratinocytes
