摘要
目的:通过血管铸型技术和数字减影造影技术,观察大鼠脊髓血供来源,确立最佳的夹闭部位,以此建立和鉴定脊髓缺血再灌注损伤模型。方法:实验于2006-08-30在福建中医学院骨伤系创伤修复与重建实验室完成。实验材料:选用20只清洁级SD大鼠,体质量490～530g,雌雄各半,由上海实验动物中心提供。大鼠单笼饲养在恒温(20±2)℃,恒湿(50±5)%,人工光照明暗各12h的安静环境中,自由进食进水。实验过程中实验动物处置符合实验动物伦理学标准。实验方法:运用血管铸型技术制作大鼠动脉铸型标本,观察脊髓血供情况,确立最佳的夹闭部位。采用Zivin法改进复制模型,在最佳的夹闭部位夹闭腹主动脉制作脊髓缺血再灌注损伤模型,使用数字减影心血管机观察缺血前、缺血后、再灌注3个不同时间段的摄像,并比较分析,确定脊髓缺血完全,镜下摄像以鉴定模型成立。结果:20只大鼠均进入结果分析。从大鼠血管铸型结果看出,脊髓血供来自腰动脉,从右肾动脉上夹闭腹主动脉可以阻断2～5腰动脉,造成脊髓广泛缺血,为最佳夹闭部位;数字减影技术显示,取右肾动脉上夹闭腹主动脉制作的模型可以造成脊髓的完全缺血,鉴定该方法建立的模型成立。结论:取右肾动脉上夹闭腹主动脉为最佳部位复制缺血再灌注损伤模型的部位,该方法复制缺血再灌注模型操作简便、安全可靠。
AIM： By vessel cast technique and digital subtraction angiography （DSA）, rat spinal blood supply is observed to define the optimal clipping position and construct and identify spinal cord ischemia/reperfusion injury models. METHODS： The experiment was performed at the Laboratory of Trauma Repair and Reconstitution of Department of Orthopaedics and Traumatology of Fujian University of Traditional Chinese Medicine on August 30% 2006. Totally 20 clean-grade SD rats with the body mass of 490-530 g of either sex （half and half） were offered by Shanghai Experimental Animal Center. The rats were fed in single cage at （20±2） % constant temperature and （50±5）% constant humidity with bright-dark （each 12 hours） silent condition, freely drinking and eating. Animal intervention met the experimental animal ethical standard. Arterial corrosive casts were constructed by vessel cast technique to observe spinal blood supply and define optimal clipping position. Duplicating models were improved by Zivin method. Abdominal aorta was occluded at optimal clipping position to make spinal cord ischemia/reperfusion injury models. These models were identified by DSA before and after ischemia, during reperfusion. RESULTS： Totally 20 rats were involved in the result analysis. Rat vessel cast results showed that spinal cord blood was from lumbar artery. Clamping abdominal aorta at right renal artery could block 2-5 lumbar arteries, resulting in spinal cord ischemia, which was the optimal clipping position. DSA showed that models made by clamping abdominal aorta at right renal artery could cause fully spinal cord ischemia and verified that these models were constructed successfully. CONCLUSION： Clamping abdominal aorta at right renal artery is the optimal clipping position to duplicate ischemia/reperfusion injury models. This method is convenient, safe and reliable for establishing ischemia/reperfusion injury models.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第40期8193-8195,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
福建省高层次人才科研基金资助(1401)
国家自然基金项目(30572401)
福建省自然基金项目(C0510023)~~
