摘要
目的:观察脊髓分级缺血再灌注损伤与行为学变化的关系。方法:40只新西兰大白兔随机分为假手术组、缺血30min组、缺血45min组和缺血60min组,每组10条。采用肾下腹主动脉阻断法,建立脊髓缺血再灌注损伤模型。手术后联合采用Reuters法、Jacobs法和Rivlin法对后肢感觉、运动、和反射功能综合评估。术后第2天HE染色观察腰髓病理学改变。结果:阻断腹主动脉血流30、45,60min后开放表现出轻、中、重不同程度缺血再灌注损伤脊髓的病理变化特点。脊髓轻度缺血再灌注损伤后神经功能评分恢复良好且迅速;脊髓中度缺血再灌注损伤后神经功能评分恢复缓慢且不能完全恢复;脊髓重度缺血再灌注损伤后神经功能评分不能够恢复至术前水平。结论:联合采用Reuters法、Jacobs法和Rivlin法能够准确地反映脊髓分级缺血再灌注损伤后行为学变化。
Objective： To observe the relationship between graded spinal cord ischemia/reperfusion injury and the changes of ethology. Methods 40 rabbits were equally randomized into the sham - operation group, ischemia for 30min, 45min and 60min group. The spinal cord ischemia- reperfusion model was made by clamping the infrarenal aortic in rabbits. The sensory, motor and reflex functions of hind extremities were evaluated by Reuters, Jacobs and Rivlin method in combination after reperfusion. After 2 days of reperfusion, the pathological changes of lumbar segmental spinal cord were observed with HE staining. Results The pathological characters with mild, moderate and severe spinal cord ischemia/reperfusion injury can be simulated by declamping after 30min, 45min and 60min infrarenal aortic cross - clamping. It showed that after spinal cord was mild injured during ischemia/ reperfusion neurologic function score （NFS） recovered rapidly. NFS recovered slowly and incompletely during moderate spinal cord ischemia/reperfusion. NFS could not restore to the level of preischemia during severous spinal cord ischemia/reperfusion injury. Conclusions Reuters, Jacobs and Rivlin method in combination can be a index for the changes of ethology during graded spinal ischemia/repeffusion injury.
出处
《华西医学》
CAS
2007年第3期573-575,共3页
West China Medical Journal
基金
国家教育部高等学校博士学科点专项科研基金资助项目(20030610087)
