摘要
目的:观察白细胞介素-1α(interleukin-1α,IL-1α)对体外培养的人眼小梁细胞(human trabecular meshwork cell,HTC)增殖和形态结构的影响。方法:取人眼小梁组织进行小梁细胞体外培养,取传3～5代的人小梁细胞,加入不同浓度的IL-1α的DMEM/F12液,通过比色法检测IL-1α对培养的人小梁细胞增殖的影响;用倒置相差显微镜、光镜、和电镜观察细胞形态结构的变化。结果:⑴MTT测定法:阴性对照组与低浓度实验组(0.5,1μg/L)相比,吸光度值无显著差异(P>0.05),IL-1α≥10μg/L组与对照组相比,吸光度值有显著差异(P<0.05),与对照组比较,0.5μg/L浓度的IL-1α对人小梁细胞形态结构差异无显著性;IL-1α浓度进一步增加,作用时间延长,细胞的损伤也加重。结论:IL-1α≥10μg/L时,将破坏细胞形态结构并影响其功能,而且显著抑制体外培养的人小梁细胞的增殖。
AIM：To investigate the effects of interleukin-1α（IL-1α）on the proliferation and morphology of cultured human trabecular meshwork cells（HTCs）. METHODS：Human trabecular meshwork cells were culturedin vitro The effects of IL-1α on the proliferation of HTCs were detected with MTT staining colorimetry.The three-to five-generation HTC were treated with different concentrations of IL-1αin DMEM/F12 medium.Cellular morphology was observed by inverted phase-contrast microscope,light microscope and electron microscope. RESULTS：⑴MTT staining colorimetry ：There was no significant difference of proliferation inhibition or optical density between control group and the group treated with IL-1α at lower concentration（0.5,1μg/L）（P〉0.05）;IL-1α significantly inhibited the proliferation of HTC cultured at conentration 10μg/L（P〈0.05 ）.There was no significant difference of morphology between control group and the group treated with IL-1α at concentration of 0.5μg/L.The damage appeared more severe if the concentration of IL-1α was higher or action time lasted longer. CONCLUSION：HTCs may be destroyed structurally and their function was impaired by exposure to IL-1α whose concentration is over 10μg/L.IL-1α≥10μg/L remarkably inhibited the proliferation of HTCs culuredin vitro.
出处
《国际眼科杂志》
CAS
2007年第5期1291-1293,共3页
International Eye Science
关键词
人小梁细胞
白细胞介素-1Α
炎症因子
human trabecular meshwork cell
interleukin-1α
inflammatory cytokines
