摘要
在原核生物发育分化的分子生物学研究中,以枯草杆菌为模式系统进行了许多研究,但其生命周期特别是分化过程比较简单,不象链霉菌有基质菌丝、气生菌丝、菌丝分隔形成多细胞,然后断裂形成单孢子的过程。链霉菌的这一性状与真核生物丝状真菌的分化相似。因此在原核生物中,链霉菌是研究分化基因表达调控的良好模式材料,为在时空上研究发育分化基因的多水平调控提供了有利条件。 链霉菌的发育调控启动子(P_(TH270))是国际上首先克隆的两个启动子之一。一些研究结果表明该启动子依赖于链霉菌分化关键基因——whiG。虽然该启动子的序列已被测定,启动子的-10区和-35区已被精确定位,但该启动子在链霉菌发育分化中是如何调控的,当它以高拷贝数的形式存在时对细胞内含物的组份有何影响,这种影响怎样与分化关联,这些都是有待解决的重要问题。
758bp of promoter——PTH270was subcloned into Streptomyces plasmid pIJ4083.pIJ4471 was then obtained after recombinant plasmid was introduced into Streptomyces coelicolor J1501. Streptomyces coelicolor J1501 / pIJ4083 and J1501 / pIJ4471 were grown on minimal medium (MM)agar containing mannitol and 7μg / ml thiostrepton for 4 days, and ultrathin sections of colonies were stained with silver proteinate to demonstrate glycogen and observed in the electron microscope. Result indicated that the glycogen was produced by J1501 /pIJ4083, whereas glycogen was little produced by J1501 / pIJ4471. Also dark spores were not observed in J1501 / pLB471 with lead stain in contrast with dark spores which wasproduced by J1501 / pIJ4083, suggesting that promoter——PTH270was possibly related toStreptomyces differentiation.
出处
《微生物学报》
CAS
CSCD
北大核心
1997年第1期58-61,共4页
Acta Microbiologica Sinica
基金
中国科学院基金资助项目
英国皇家学会合作研究项目的部分工作。
