摘要
目的探讨脊髓P2X4受体在链脲菌素诱导的糖尿病大鼠神经病理性痛中的作用。方法雄性Wistar大鼠84只,3月龄,体重180～220 g,随机分为2组（n=42）：对照组（C组）和糖尿病神经病理性痛组（D组）。C组单次腹腔注射等体积（3.25 ml/kg）枸橼酸缓冲液,D组单次腹腔注射链脲菌素65 mg/kg制备糖尿病模型。2组分别于给药前1 d（基础值）、给药后3、7、14、21、28 d各随机选取7只大鼠,测定机械缩足阈值和热痛阈潜伏期,痛阈测定后断头处死大鼠,取L（4,5）脊髓组织,用RT-PCR方法测定P2X4受体mRNA表达水平。结果与C组相比,D组脊髓P2X4受体mRNA表达上调,机械缩足阈值降低（P〈0.05）,热痛阈潜伏期差异无统计学意义（P〉0.05）;与基础值比较,D组给药后14 d机械缩足阈值逐渐降低,给药后28 d时达最低值;给药后3 d脊髓P2X4受体mRNA表达逐渐上调,给药后7d达峰值（P〈0.05）。结论脊髓P2X4受体表达上调参与了糖尿病大鼠神经病理性痛的发生。
Objective To investigate the role of P2X4 receptor in the spinal cord in diabetes neuropathic pain induced by streptozocin. Methods Eighty-four male Wistar rats weighing 180-220 g were randomly divided into 2 groups （ n = 42 each） ： control group and diabetic neuropathic pain group （ D）. Diabetes was induced by intraperitoneal streptozocin 65 mg /kg in group D while the animals in control group received equal volume of buffered saline instead. Blood glucose concentration was measured 2 days later. Any rats in group D with blood glucose 〈 16.0 mmol/L were rejected from the study. The paw withdrawal threshold to mechanical stimulus （MWT） and the response of the tail to a thermal nociceptive stimulus （TFL） were measured 1 day before （baseline） and 3 d, 7 d, 14 d,21 d and 28 d after IP streptozocin injection （ n = 7 each）. The animals were then killed and the lumbar segment （ L4,5 ） of the spinal cord was removed for determination of P2X4 receptor mRNA expression using RT-PCR. Resttlts The MWT was significantly decreased and the P2X4 receptor mRNA expression in the spinal cord was significantly increased after IP streptozocin injection as compared with the baseline values before streptozocin injection in group D. The MWT was significandy lower and P2X4 receptor mRNA expression in the spinal cord was significantly higher in group D than in control group. Conclusion The P2X4 receptor in the spinal cord is involved in the development of diabetic neuropathic pain in rats.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2007年第9期799-802,共4页
Chinese Journal of Anesthesiology
基金
湖北省教育厅B类资助项目（B200524003）
关键词
受体
细胞表面
脊髓
糖尿病神经病变
神经痛
Receptom, cell surface
Spinal cord
Diabetic neuropathies
Neuralgia
