摘要
目的:研究KLF6基因在前列腺癌中的遗传状况。方法:应用DHPLC技术、LOH和直接测序的方法,检测了320例局限性前列腺癌和28例有远处转移的前列腺癌标本中KLF6基因的突变。结果:采用DH-PLC方法在3组(245例)前列腺癌标本中筛选有异常信号的标本,然后直接测序,未发现KLF6基因的突变;将其中55例标本行LOH分析,发现6例阳性标本,亚克隆后直接测序也未发现有KLF6的突变。在另外75例标本和28例转移性前列腺癌标本中分别发现1例纯合性和杂合性体细胞突变。KLF6基因在前列腺癌中突变率很低。结论:KLF6基因突变在前列腺癌中很少见,对其在前列腺癌中作用有待于进一步的研究。
OBJECTIVE： To study the mulations and clinicopathological relevance of KLF6 gene in prostate cancer. METHODS： Denaturing HPLC （DHPLC） analysis, direct sequencing and/or LOH approaches were used to detect KLF6 mutations in prostate cancer specimens, RESULTS： In an effort to assess the clinicopathological significance of KLF6 mutations in prostate cancer, genomic DNA for mutations in exon 2 of the KLF6 gene was screened in 320 primary prostate tumors and 28 metastatic tumor samples by using Denaturing HPLC （DHPLC） analysis and/or by direct sequencing approach. Other than one somatic mutation identified in the analysis of 28 metastatic tumors and one germ-line mutation in one non-metastatic tumor, no any somatic mutations of KLF6 was detected in the 320 primary non-metastatic prostate tumors. CONCLUSION： Klf6 gene mutations are rare in patients with primary prostate cancer.
出处
《中华肿瘤防治杂志》
CAS
2007年第20期1559-1562,共4页
Chinese Journal of Cancer Prevention and Treatment
关键词
前列腺肿瘤
基因
抑制
肿瘤
突变
prostatic neoplasms
genes, suppression, tumor
mutation
