摘要
目的克隆家族性急性髓系白血病相关新基因全长,在分子水平上探讨急性白血病发生发展的机制。方法以构建的家族性急性髓系白血病抑制性消减性文库中1个有差异表达的新基因 EST 序列(zywb4)为基础,综合应用电子克隆和 cDNA 末端快速扩增法(RACE)技术克隆家族性急性髓系白血病相关新基因的全长 cDNA。结果获得家族性急性髓系白血病差异表达新基因 ELF2C的全长 cDNA 和432 bp 的开放阅读框(ORF),Blast 检索为一功能未知基因,被 GenBank 收录,注册号为 DQ359746。结论成功获得一个家族性急性髓系白血病相关新基因 ELF2C cDNA 全长,为进一步研究其功能提供了依据。
Objective To clone the full-length cDNA of a novel gene of familial acute myelogenous leukemia and to explain the molecular mechanism of the disease at the gene level. Methods Based on a EST sequence （zywb4） from a subtractive cDNA library of specially or differentially expressed genes constructed in familial acute myelogenous leukemia, electronic cDNA cloning and SMART-rapid amplification of cDNA ends （SMART- RACE） were used to clone the full-length cDNA of a novel associated gene of familial acute myelogenous leukemia. Results One sequence of 2257 bp was obtained, which obeyed Kozak rules, and contained an open reading frame （ORF） and a polyA tail. BLAST analysis showed that it may be a novel gene. The new sequence was submitted to GenBank with the accession number： DQ359746 and designated as ELF2C. Conclusion A full- length cDNA of a novel gene （ELF2C） related to familial acute myelogenous leukemia is obtained, which is helpful to further investigation of its function in familial acute myelogenous leukemia.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2007年第32期2245-2248,共4页
National Medical Journal of China
基金
福建省重大科研项目资助课题(2003F003)
关键词
系谱
白血病
粒细胞
急性
基因
克隆
RACE
Pedigree
Leukemia, granulocyte, acute
Gene
Clone
Rapid amplification of cDNA ends
