摘要
对酶转化法制备L-肉碱的菌种选育、工艺优化、合成代谢途径及下游技术等方面的研究进展进行综述,并由此提出,提高以巴豆甜菜碱为前体的酶转化法的转化率、获得L-肉碱和未转化巴豆甜菜碱高效、经济的分离技术、消减以γ-丁基甜菜碱为前体的培养基成本并缩短转化时间以及寻找廉价、与L-肉碱物化性质差异较大的前体物质以减少产品纯化费用是今后酶转化法制备L-肉碱研究中应重点解决的问题。
The progress in research of production of L- carnitine by the method of bioconversion from achiral precursors was reviewed, including the screening of bacterial strains, the optimization of process, the metabolic pathways in biosynthesis and the downstream technology, etc. Taken together, the main problems and future direction in research was proposed and run as follows： （1） To bioconversion from crotonobetaine, it＇s essential to enhance the conversion rate and acquire more economical and efficient techniques of separating L- carnitine from crotonobetaine. （2） To bioconversion from y-butyrobetaine, it＇s essential to reduce the cost of culture media and the time of a bioconversion cycle. （3） To reduce the cost of separation and purification of product, it＇s essential to acquire cheap precursors whose physical or chemical properties far from L- carnitine.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2007年第8期118-121,共4页
Food and Fermentation Industries
基金
中国农业科学院二缀岗位杰出人才基金
关键词
L-肉碱
制备
酶转化法
研究进展
L- carnitine, production, bioconversion, research progress
