摘要
为探索生物材料低温保存的新方法,以质量分数为0.9%的KMnO4溶液为实验样品,将其置于-30℃低温环境中冷冻,同时施加交变电场(频率为50 Hz^100 kHz)干扰,通过显微观察研究交变电场对含盐溶液液-固相变过程的影响.研究发现,交变电场作用对溶液中冰晶生长具有明显的抑制作用,形成的纯水冰晶体积明显减小,离子分布趋于均匀,部分区域可能形成微晶冰或含盐冰.分析认为,对于强电解质溶液,交变电场作用下产生的离子扰动是影响含盐水溶液液-固相变过程的主要原因.
Ice formation inside or outside cells is revealed as a factor causing cryo-injury to cells/tissues during cryo-preservation. It is confirmed by test that alternating electric fields is able to significantly influence ice formation at a relatively slow cooling rate. （mass fraction） A solution with 0. 9% mass fraction of KMnO4 as test sample was cooled under alternating fields ranging from 50 Hz to 100 kHz at -30℃. The control sample is similarly cooled without electric fields. Simultaneously morphology investigation is carried out, and it is found that alternating fields ex- ert obvious effects to inhibit ice crystal growing. Thus the ion disturbance can be regarded as the major factor to influence ice crystal growing. The bulk of pure ice crystal （without salt ion） is significantly reduced, and salt ion distribution in whole area tends uniformly. In some regions, microcrystalline ice crystal or ice crystal with salt ion mav he formed.
出处
《西安交通大学学报》
EI
CAS
CSCD
北大核心
2007年第10期1233-1236,1241,共5页
Journal of Xi'an Jiaotong University
基金
国家自然科学基金资助项目(50307005)
关键词
KMnO4溶液
相变
交变电场
KMnO4 solution
phase transition
alternating electric fields
