摘要
在好氧条件下,从长期受硝基苯污染的化工厂排污口底泥和河道底质中分离得到3株对硝基苯有明显降解作用的优势微生物。为了比较这些优势菌的降解效果,作了硝基苯初始质量浓度分别为50mg/L、25mg/L、12.5mg/L、6.25mg/L、3.13mg/L等不同梯度下的模拟修复实验,并且检验了单一菌与混合菌的降解能力。实验结果表明:当硝基苯初始质量浓度为50mg/L时,优势菌作用不明显,硝基苯的去除主要依靠自然挥发作用;当硝基苯初始质量浓度为3.13mg/L时,混合菌对硝基苯的降解能力较强,72h的去除率达到99%,而此时空白对照(CK)的去除率仅为60%。分析实验结果得到,混合菌可能存在协同作用;由于产物中未检出NO2-,所以硝基苯降解途径中可能存在部分还原历程。
This paper is aimed at presenting its authors＇ study on the possibilities to apply the biodegradation feature of nitrobenzene （NB） to restoration of the contaminated river water quality. For its research purpose, 3 aerobic strains were isolated and chosen from the sludge of chemical factory effluent and some soil samples from a NB contaminated river water. After that, we selected 5 different initial NB concentrations, that is, 50 mg/L,25 mg/L,12.5 mg/L,6.25 mg/L, 3.13 mg/L for our separately done simulation remedying experiments to check the degrading effects. And finally we investigated different degrading capabilities of the single as well as the mixed strains. Results of our simulated experiments show that, when the initial concentration of NB was 50 mg/L, the effect of degrading strains was insignificant, with its NB removal capability mostly relying on volatilization. But, when the initial concentration of NB was 3.13 mg/L, the mixed strains were found to have prominent degrading capabilities, whose NB, and NB removal rate could reach 99% within 72 hours. At the same time, the control check （CK） of the NB removal rate was only 60% . Based on the previous experimental results, the mixed strains can be endowed with the cooperated effects. Furthermore, because of the absence of NO2^- , it is likely to promote the undergoing of the portion reduction process with the NB degrading process going on.
出处
《安全与环境学报》
CAS
CSCD
2007年第5期65-67,共3页
Journal of Safety and Environment
基金
辽宁省教育厅青年基金项目(2005264)
中国科学院知识创新工程重要方向项目(KZCXZ-YW-402)
关键词
环境生物学
硝基苯
降解菌
好氧
筛选分离
environmental biology
nitrobenzene
nitrobenzene degrading strain
aerobic
screening and isolation
