摘要
鉴定了从处理硝基苯废水的人工湿地中分离出的一株降解硝基苯的优势菌yc11-1,并采用中心复合设计法优化影响菌株降解硝基苯的因素来提高菌株对硝基苯的降解率。经形态学和菌株的16S rDNA序列分析,菌株yc11-1为蜡质芽孢杆菌(Bacillus cereus)。该菌株降解硝基苯的最佳条件为初始硝基苯浓度84.84 mg/L、菌种投加量12.97%、培养温度29.4℃和pH 7.1,在此条件下理论降解率可达91.14%,实际降解率为90.58%。
A nitrobenzene-degrading strain isolated from a nitrobenzene-irrigating artificial wetland was identified, and its optimal degradation condition was studied. The strain was identified by its morphological characteristics and 16 S rDNA fragment. Central composite design(CCD) was employed to optimize the nitrobenzene degrading condition. The strain yc11-1 was identified as the specie of Bacillus cereus, and its optimal degradation condition was 84.84 mg / L initial nitrobenzene, 12.97%inoculation size, incubation in 29.4 ℃ and pH 7.1. Under this condition, the predicated maximum removal efficiency of nitrobenzene was 91.14%, and the realistic efficiency was 90.58%.
出处
《湖北农业科学》
北大核心
2013年第16期3824-3826,3855,共4页
Hubei Agricultural Sciences
基金
江苏省自然科学基金项目(BK2009171)
江苏省滩涂生物资源与环境保护重点建设实验室开放项目(JLCBE09005)
关键词
硝基苯
蜡质芽孢杆菌
降解
中心复合设计
nitrobenzene
Bacillus cereus
degradation
central composite design
