摘要
采用乙醇沉淀,在抗坏血酸和还原铁粉、50℃水浴的条件下,富血红素多肽酶解液及其超滤分离组分(透过液和截留液)以2:1比率(v/m)分别与FeCl2反应1 h得总铁含量为127.34 mg/g,128.70 mg/g和47.83 mg/g的多肽螯合铁。红外光谱分析表明:多肽螯合铁形成新的-C≡N特征吸收峰,证明了多肽与铁形成新的化学结构。脂肪储藏实验表明:在促进脂肪过氧化值升高方面,富血红素多肽螯合铁比富血红素多肽酶解液和透过液要强,但比FeCl2弱。
The enzymatic hydrolysate, which was rich in ferrous peptides, were reacted with FeCI2 at a ratio of 2：1 in a water bath at 50℃ for 1 h in the presence of antioxidants, then the mixtures were centrifuged at 15000 r/min for 10 min and the supematant was precipitated by ethanol. The precipitate was washed several times by ethanol, followed by a vacuum dry. Ferrous peptides were obtained with content of total iron being of 127.34 mg/g （heme-enriched peptide）, 128.70 mg/g （permeate peptide） and 47.83 mg/g （retentate peptide）, respectively. The structure of the new compound was identified by Infrared spectrometry. It was also found that oxidation stability of ferrous heme-enriched peptide was higher than that of FeCl2 but lower than that of heme-enriched peptide or permeate peptide.
出处
《现代食品科技》
EI
CAS
2007年第10期1-3,6,共4页
Modern Food Science and Technology
基金
广东省科技计划项目"粤港关键领域重点突破招标项目"(2005A20303003)
广东省自然科学基金项目(031348)
