摘要
目的:建立以高效液相色谱法测定五维B颗粒中维生素B1含量的方法。方法:色谱柱为YWG-C18,流动相为1%冰醋酸溶液(含0.005mol.L-1庚烷磺酸钠)-甲醇-乙腈(65∶21∶14),流速为1.0mL.min-1,检测波长为254nm,进样量为5μL。结果:维生素B1检测浓度的线性范围为0.1～0.6mg.mL-1(r=0.9997);平均回收率为100.3%,RSD=0.36%。结论:本方法简便、快速、准确、专属性强,可用于该制剂中维生素B1的含量测定。
OBJECTIVE： To establish HPLC method for the determination of VB1 in five vitamin -granules. METHODS： YWG- C18 column was used. The mobile phase was dilute glacial acetic acid containing 0.005mol· L^-1 sodium heptanesul-fonate- methanol - acetonitrile（65 ： 21 ： 14） with a flow rate of 1.0mL·min^-1. The detection wavelength was set at 254nm and the sample size was 5μL. RESULTS： The calibration curve of VB1 was linear in the range of 0.1-0.6mg· mL^-1（r = 0.999 7） . The average recovery was 100.3% （RSD = 0.36%） . CONCLUSION： The method was proved to be simple, rapid, accurate and specific, and it can be used for the quantitative determination of VBI in five vitamin- granules.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第28期2211-2212,共2页
China Pharmacy
