摘要
目的建立稳定的大鼠肝移植动物模型。方法参考Kamada二套袖法并稍作改进,预实验后,双人行大鼠原位肝移植50例。结果手术成功率(存活24 h以上)90%,2周存活率78%。结论熟练的外科技术、默契配合、标准化的手术操作以及手术细节的完善程度是模型成功与否的关键。
Objective To establish a reliable model of orthotopic liver transplantation in rats. Methods After some rehearsal, 50 procedures of rat orthotopic liver transplantation were performed by using modified Kamada＇s two - cuffed technique. Results The 24 - hour and 2 - week survival rates of the model rats were 90% and 78% respectively. Conclusion Delicate and skillful surgical manipulation, smooth collaboration of the operating team, standardized protocol and some detailed modifications are the key to the success of rat liver transplantation.
出处
《徐州医学院学报》
CAS
2007年第9期588-591,共4页
Acta Academiae Medicinae Xuzhou
基金
江苏省自然科学基金(BK2007057)
关键词
肝移植
大鼠
模型
移植
liver transplantation
rat
model
transplantation
