摘要
目的探讨糖皮质激素的应用在大鼠肝移植模型建立中的意义。方法将200只SD大鼠采用简单随机化法随机分为实验组和对照组两组,实验组术前30 min肌肉注射硫酸阿托品溶液0.1 mg/kg、头孢唑啉钠0.3 g/kg及氢化可的松5 mg/kg,对照组术前30 min肌肉注射硫酸阿托品溶液0.1 mg/kg、头孢唑啉钠0.3 g/kg及与激素等量的生理盐水,之后操作步骤2组相同,比较2组间供体手术时间、修肝时间、受体手术时间、无肝期以及7 d内的大鼠存活率。结果实验组和对照组间的供体手术时间、修肝时间、受体手术时间及无肝期比较差异均无统计学意义(P>0.05),而实验组术后第1 d、第3 d、第7 d的成活率均分别明显高于对照组〔96%(48/50)比80%(40/50),P<0.05;92%(46/50)比72%(36/50),P<0.05;90%(45/50)比54%(27/50),P<0.05〕。结论糖皮质激素的使用可能有助于提高肝移植模型大鼠的成活率。
Objective To explore significance of glucocorticoid for rat liver transplantation model. Methods Two hundred rats were randomly divided into experimental group and control group. The experimental group rats were injected with sulfate atropine resulotion 0.1 mg/kg, cephazolin-Na 0.3 g/kg and hydrocortisone 5 mg/kg while the control group rats were injected with sulfate atropine resulotion 0.1 mg/kg, cephazolin-Na 0.3 g/kg and equal volume of normal saline with glucocorticoid at 30 min before operation. The donor surgery time, repairing liver time, recipient surgery time, anhepatic phase, and 1-day, 3-day and 7-day survival rates were compared between these two groups. Results The donor surgery time, repairing liver time, recipient surgery time, and anhepatic phase had no significant differences between the experimental group and control group（P0.05）, while the 1-day, 3-day and 7-day survival rates of the experimental group were significantly higher than those of the control group〔 96%（48/50） versus 80%（40/50）, P〉0.05; 92%（46/50） versus 72%（36/50）, P0.05; 90%（45/50） versus 54%（27/50）, P〈0.05〕. Conclusion Usage of glucocorticoid might contribute to improve survival rate of rat liver transplantation model.
出处
《中国普外基础与临床杂志》
CAS
2016年第5期552-555,共4页
Chinese Journal of Bases and Clinics In General Surgery
基金
省应用基础研究项目(编号:2013FB191)~~
关键词
大鼠
肝移植
糖皮质激素
Rat
Liver transplantation
Glucocorticoid
