摘要
目的:探讨环氧化酶-2基因(COX-2)的两个功能性单核苷酸多态(Single nucleotide polymorphisms,SNPs)与胃癌易感性的关系。方法:采用病例-对照研究设计,研究对象为254例经组织学确诊的胃癌病例和304例按年龄、性别进行匹配的无肿瘤史的社区对照人群。采用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)测定COX-2基因-1195G/A多态,错配聚合酶链反应-限制性片段长度多态性方法(PIRA-PCR)测定COX-2基因8473T/C多态的基因型,比较不同基因型与胃癌风险的关系,并探讨不同环境因素在其中所起的影响。结果:COX-2-1195GG、GA和AA三种基因型以及8473TT、TC和CC三种基因型在病例组和对照组间的频率分布无统计学意义。Logistic回归分析也未发现COX-2 SNP-1195G/A以及SNP8473T/C与胃癌的风险度关联有统计学意义(SNP-1195 GA+AA:校正OR=1.09,95%CI=0.70-1.67;SNP8473 TC+CC:校正OR=1.18,95%CI=0.83-1.69)。结论:COX-2-1195G/A和8473T/C多态性可能与胃癌易感性无关,尚需进一步大样本量的研究予以证实。
Objective：To explore the correlation of two functional nucleotid epolymorphisms in the cyclooxygenase-2（COX-2）gene with susceptibitity to gastric cancer in a Chinese population of Yixing city.Methods： Polymerase chain reaction-restricted fragments length polymorphism（PCR-RFLP）and the primer-introduced restriction analysis（PIRA-PCR）assay was used to defect genotype of the COX-2-1195G/A and 8473T/C polymorphisms,respectively.In a case-control study,254 gastric cancer cases and 304 cancer- free controls were included in a Chinese population of Yixing city.Results：There was no statistical difference in the genotype frequencies of these two polymorphisms between the cases and controls（P=0.695 for-1195G/A and P=0.762 for 8473T/C）. Logistic regression analyses showed that both variant genotypes of COX-2-1195 and COX-2 8473 polymorphisms were not significantly associated with susceptibility to gastric cancer（adjusted OR=1.09,95% CI=0.70-1.67 for SNP-1195 GA＋AA and adjusted OR=1.18,95% CI=0.83-1.69 for SNP8473 TC＋CC）.Conclusion：These findings suggested that these two COX-2 polymorphisms may not be associated with genetic susceptibility to gastric cancer.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第8期890-894,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(30271148和30571605)
江苏省社会发展资助项目(BS2004510)
关键词
胃癌
COX-2基因
单核苷酸多态性
遗传易感性
gastric cancer
cyclooxygenase-2
single nucleotide polymorphisms
susceptibility
