摘要
目的探讨HLA—A、HLA—B、HLA—DRB1位点基因与山西汉族激素抵抗肾病综合征（SRNS）的相关性。方法用聚合酶链反应序列特异性引物法，对30例SRNS患者（成人22例，儿童8例）和45例正常对照者进行了HLA—A、HLA—B、HLA—DRB1等位基因分型，并分析了A、B、DRB1基因在各组的分布。结果SRNS患者组HLA—B＋15、B＋44基因频率较正常对照组增高（P〈0．05）；成人SRNS患者组HLA—DRB1＊07、B＊44基因频率较正常对照组增高（P〈0．05），成人SRNS患者组HLA—DRBl}15基因频率较正常对照组降低（P〈0．05）；儿童SRNS患者组HLA—DRB1＊10基因频率较正常对照组增高（P〈0．05）。结论SRNS发病可能与HLA—B＊15、B＋44基因有关，成人SRNS发病可能与HLA-DRB1＊07、B＊44基因有关，HLA—DRB1＊15对成人SRNS发病可能有保护作用，儿童SRNS发病可能与HLA—DRB1＊10基因有关；HLA与SRNS的相关性不仅与人种、国家和地区有关，还可能与发病年龄有关。
Objective To investigate the predisposing alleles of HLA-A, HLA-B and HLA-DRB1 genes in Han chinese with steroid-resistant nephrotic syndrome （SRNS）. Methods Polymerase chain reaction-spe- cific sequence primers （PCR-SSP） method was used to type HLA-A, HLA-B and HLA-DRB1 alleles in 30 patients with SRNS, which includes （22 adults and 8 heathy children） , and 45 control of heathy Han chineses from Shanxi province. Results The allele frequencies of HLA-B ＊ 15 and HLA-B ＊ 44 were higher in SRNS patients than those in normal controls （ P 〈 0.05 ）. The allele frequencies of HLA-DRB1 ＊ 07 and HLA-B ＊ 44 were higher in adults with SRNS than those in normal controls（ P 〈 0.05 ） . The allele frequency of HLA-DRB1 ＊ 15 was lower in adults with SRNS than those in normal controls（ P 〈 0.05 ） and the allele frequency of HLA-DRB1 ＊ 10 was higher in children with SRNS than those in normal controls（P 〈0.05 ）. Conclusion The results suggest that there may be association between SRNS and HLA-B ＊ 15, HLA-B ＊ 44, between adults with SRNS and HLA-DRB1 ＊ 07 and HLA-B ＊ 44 and between children with SRNS and HLA-DRB1 ＊ 10. Possibly HLA-DRB1 ＊ 15 allele plays a protect role in adults with SRNS and HLA associations with SRNS is not only related with area and race but also with age of disease onset.
出处
《国际免疫学杂志》
CAS
2007年第5期284-287,共4页
International Journal of Immunology
