摘要
本文报道利用多聚酶链反应（ＰＣＲ）技术及特异性寡核苷酸（ＳＳＯ）探针检测了１００例原发性非ＩｇＡ系膜增殖性肾炎（Ｎｏｎ－ＩｇＡＭｓＰＧＮ）患者ＨＬＡ－ＤＲ等位基因频率，并与２５５例中国北方汉族正常人进行了比较，发现ＨＬＡ－ＤＲ１２（５）等位基因频率在Ｎｏｎ－ＩｇＡＭｓＰＧＮ患者明显高于正常人（２７．０ｖｓ７．８，Ｐｃ＜０．０１）。并从免疫遣传学角度对ＨＬＡ与Ｎｏｎ－ＩｇＡＭｓＰＧＮ的关联进行了探讨。
he HLA-DR alleles in 100 cases of patients with mesangial proliferative glomerulonephritis(MsPGN and255 random healthy northern Chinese donors were determined by using the polymerase chain reaction(PCR) and aset of 30 sequence-specific oligonucleotide(SSO)probes which identified 33 different DRB alleles.The most commonallele were DR2(21.2%),DR7(14.5%)and DR9(14)in the northern Chinese donors while a significantly high-er frequency for DR 12(5) were found in MsPGN.It is obvious that HLA-DR 12(5) is implicated in the genetic sus-cep-tibility to MsPGN in Chinese population.
出处
《中华微生物学和免疫学杂志》
CSCD
北大核心
1994年第6期419-422,共4页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金
关键词
基因频率
系膜增殖性
肾炎
寡核苷酸探针
HLA-DR
Gene frequency
Mesangial proliferative glomerulonephritis
Oligonucleotide
