摘要
目的建立高通量、可定量中和抗体检测方法,并且调查广州健康成人及儿童人群中人5型腺病毒中和抗体存在情况。方法采用以带有分泌型碱性磷酸酶报告基因的5型重组腺病毒使用化学发光法,调查116份成人血清和94份儿童血清中5型腺病毒中和抗体情况。结果该方法是一种适合高通量调查的血清学调查方法,广州地区成人中人5型腺病毒中和抗体阳性比例占26.72%,儿童中阳性比例为17.02%。结论5型腺病毒在广州人群中具有一定的免疫程度,结合中和实验结果来应用该种病毒载体并同时开发其他血清型的腺病毒载体很有必要。
Objective To establish a high-throughput chemiluminescence assay of serotype 5 specific neutralizing antibody and understand the epidemiology of this antibody in the healthy adults and children in Guangzhou. Methods Using rAd5 carrying the reporter gene of secreted alkaline phosphatases （SEAP）, serum samples from 116 healthy adults and 94 healthy children were examined with chemiluminescence assay to detect the presence of Ad5 neutralizing antibody. The reliability of this assay was tested against conventional cytopathic effect observation, Results The chemiluminescence assay using secreted alkaline phosphatases （SEAP） as the reporter allowed rapid, sensitive, specific and reproducible detection of serotype 5 specific neutralizing antibody for epidemiological study of Ad5, which was positive in 26.72% of the adults and 17.02% of the children in this study. Conclusion Ad5 neutralizing antibody is prevalent in the population of Guangzhou, suggesting the necessity of developing other serotype adenovectors for better vaccination and therapeutic effects.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第9期1323-1326,1330,共5页
Journal of Southern Medical University
基金
国家863项目(2005AA218040)~~
关键词
人5型腺病毒
化学发光法
中和试验
流行病学研究
human adenovirus type 5
chemiluminescence
neutralization assay
epidemiological study
